|
Issue: Biotechnology; Nong Lam University, Ho Chi Minh City, Vietnam.
Code: 60.42.02.01
Adviser: NGUYEN BAO QUOC (PhD., Ass. Prof.)
Hepatocellular carcinoma (HCC) is the most common primary cancer of the liver, accounting for 70% - 85% of liver cancer cases. HCC has the leading incidence and mortality rate of cancer diseases in general. All most HCC cases were detected in late stage and patients have little chance of effective treatments. Recently, the use of microRNA molecules as potential markers has been focused on the diagnosis of HCC cases and other cancer diseases. In this study, the expression levels of miRNAs between HCC and normal liver tissues provided by the microarray dataset collected from Gene Expression Omnibus (GEO) were evaluated by bioinformatics tool. Then, ten candidate microRNA molecules were selected base on previous studies and expression profiles of these miRNAs in plasma samples were evaluated with three groups of HCC, Hepatis B patients and healthy adults using real-time PCR. The results indicated that at least two microRNAs (hsa-miR-192 and hsa-miR-10a) showed a significant difference in the expression between HCC and healthy groups in tissue and plasma samples. In contrast, two microRNA including hsa-miR-193a and hsa-miR-25 showed increased expression in only HBV-infected group.
The results of combined ROC curve analysis between miRNAs showed that cluster of hsa-miR-25 and hsa-miR-10a was the best with the average AUC value of the microarray and plasma samples were 96% and 85.7% respectively, which has implications for the non-invasive marker approach and understanding of the expression of potential microRNAs, which promising for developing appropriate tools to diagnosis and prognosis of hepatocellular carcinoma in Vietnamese patients.
Keywords: Bioinfomatics, Diagnosis, Hepatocellular carcinoma, MicroRNA, Plasma
|
[ Tin tức liên quan ]___________________________________________________ -
Nguyen binh Duy. 2011. Environmental and Economic Effect of Nitrogen Use On Paddy Rice Production In Vietnam and Asia-Pacific Countries. MSc. Thesis. Hankyong National University, Korea. E-mail: duy.nb@iasvn.org ( Thứ năm, 10/11/2011 )
-
MSc. THESIS ABSTRACT By Mai Thanh Truc, 2010Email address: bamboo3182001@yahoo.com Ibaraki University, Japan. ( Thứ năm, 10/11/2011 )
-
Binh CQ NGUYEN. 2011. MSc Thesis Abstract. e-mail: ncqbinh@gmail.com Molecular and Biochemical Characterization of Germin-like Protein in Rice ( Thứ năm, 10/11/2011 )
-
MSc THESIS ABSTRACT NGUYEN NAM, 2011 [mailto:nam_nguyen87@hotmail.com] ( Thứ năm, 10/11/2011 )
-
MSc THESIS ABSTRACT Ly thi Phuong Loan. 2011 ( Thứ năm, 10/11/2011 )
-
MSci THESIS ABSTRACT By NGUYEN DUY, 2010, nguyenmduy@gmail.com, Wageningen University, The Netherlands. ( Thứ năm, 10/11/2011 )
-
Pham Thi Be Tu. 2007. Genetic diversity analysis of ninety traditional rice (Oryza sativa L.) varieties from Cuu Long Rice Research Institute genebank, Vietnam. MSc. Thesis. University of the Philippines los Banos. Philippines; 110p ( Thứ năm, 10/11/2011 )
-
Recovery of fish oils and proteins from salmon processing co-products and their potential applications. Nguyen Van Tri. 2009. ( Thứ năm, 10/11/2011 )
-
Graft-transmissible virus resistance in tobacco (master of molecular biology). The University of Queensland, Australia. Dang Minh Tam, clrri@hcm.vnn.vn; MSc. thesis 2008. Abstract ( Thứ năm, 10/11/2011 )
-
Effects of dietary energy density on the performance characteristics of finisher pigs (Tu Truong Ho, Master Thesis, Abstract) ( Thứ năm, 10/11/2011 )
-
Expression of Ezrin and Radixin in the Developing Mouse Forebrain (Bui Chi Bao - Abstract) ( Thứ năm, 10/11/2011 )
-
MSc. thesis on Genetic analysis of resistance to bacterial blight of rice (Truong Quoc Anh - Abstract) ( Thứ năm, 10/11/2011 )
-
MSc. Thesis on Plant Protection (Nguyen Huu Quang - Astract) ( Thứ năm, 10/11/2011 )
-
MSc. Thesis on Plant Biotechnology (Do Duc Tuyen - Abstract) ( Thứ năm, 10/11/2011 )
-
MSc. Thesis on Biotechnology (Tran Thi Bich Trinh - abstract) ( Thứ năm, 10/11/2011 )
-
“Improve the process for spinach (Spinacia oleracea) production hydroponically in the plastic – greenhouse” ( MSc. Nguyen Đinh Tuan, Email: tuan.nd@iasvn.org) ( Thứ năm, 15/03/2012 )
-
Survey on rice production situations and experiment to determine nitrogen doses and seeding rates for rice variety – VND99-3 in winter – spring season in Dak Nong (MSc. Vo Ngoc Vu – Email: vu.vn@iasvn.org) ( Thứ ba, 17/04/2012 )
-
The study on controlling black leaf mold on tomato (Lycopersicon esculentum Mill) in the rainy season at Cho Gao district, Tien Giang province (MSc. Chu Trung Kien – Email: Kien.ct@iasvn.org) ( Thứ ba, 17/04/2012 )
-
Bui Phuoc Tam. 2016. Responses to salinity stress in a rice (Oryza sativa L.) diversity panel at reproductive stage. MSc. Thesis Abstract. IRRI- UPLP, the Philippines. July 2016. ( Thứ hai, 15/08/2016 )
-
Effects of heat treatment on bypass of protein materials For dairy cows (Nguyen Van Phu, MSc- Email: phu.nv@iasvn.org or nguyenvanphu229@yahoo.com ) ( Thứ ba, 17/04/2012 )
|
Curently online :
9
Total visitors :
7438018 
Trở lại
In
Số lần xem: 303
