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Evaluation Of Rice Landraces In Vietnam Using SSR Markers And Morphological Characters
Thứ tư, 03-09-2014 | 12:08:14

 

Nguyen Thi Lang(1)*, Bui Phuoc Tam(1), Nguyen Van Hieu(1),Chau Thanh Nha(1), Abdelbagi Ismail(3), Russell Reinke(3) And Bui Chi Buu(2)

(1) Cuu Long Delta Rice Research Institute, Thoi Lai, Can Tho, Vietnam

(2) Institute of Agricultural Sciences for Southern Vietnam, Vietnam

(3) International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines

 

*Corresponding author’s email: ntlang@hcm.vnn.vn

 

SUMMARY

 

Information on genetic diversity among traditional varieties is critical in breeding programs as this influences parental selection in varietal development. A total of 100 traditional varieties in the genebank of the Cuu Long Delta Rice Research Institute (CLRRI), Vietnam, were used to explore this diversity using SSR markers. The study aims to evaluate the genetic diversity of traditional rice varieties and involves molecular diversity analysis using 55 polymorphic SSR markers revealed among the 100 varieties. The Vietnam varieties generated four clusters at 0.60 similarity coefficient. Some varieties with similar names were grouped into different clusters as molecular analysis showed that they were actually genetically different. The 100 landrace varieties collected were evaluated phenotypically. In the analysis of quantitative traits, the range of coefficients of variability was high. It varied from 94.38–80.3% (filled grain) to 60.02–5.63% (unfilled grain). This shows that these traits can be considered most stable as exemplified by their coefficients of variability. The highest values seen in unfilled grain indicate that this character is more affected by the environment and farmers’ cultural management practices. The mean values of quantitative trait measurements were higher (78.75–139.75 cm). The highest values noted in yield (3.10–105.16 g) and survival (21–30 days) show good prospects to plant breeders. It has remained one of the major breeding objectives in developing rice varieties. Looking at agro-morphology, ANOVA showed highly significant differences

among the 100 traditional rice varieties. The standardized Shannon-Weaver diversity indices for the quantitative morphological characters ranged from 0.68 to 0.95 with a mean of H’ = 0.79. Cluster analysis using UPGMA grouped the 100 traditional varieties into 3major clusters. Varieties collected from the same site were grouped together in the same cluster.

 

Keywords:  Coefficients of variability, molecular analysis, quantitative morphological characters, traditional varieties

 

Manuscript received: November 26, 2012; Decision on manuscript: November 22, 2013; Manuscript accepted: March 20, 2014.

© Society for the Advancement of Breeding Research in Asia and Oceania (SABRAO) 2013

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