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Using SSR markers to identify resistance gene to bacterial wilt (Ralstonia solanacearum) of tomato varieties (Solanum lycopersicum) (MSc.Thesis: Ngo Minh Dung, Email: dung.nm@iasvn.org)
Thứ tư, 22-05-2013 | 15:56:41

ABSTRACT

 

Bacterial wilt of tomato was described by Smith (1896) over 100 years ago, caused among the most serious diseases of tomato in tropical and subtropical area. It is now the major constraint on production of tomato in many parts of Vietnam. Bacterial wilt is caused by the soilborne bacterium Ralstonia solanacearum. This oomycete pathogen attacks on leaves, stems, roots of tomato. Introduction of resistant varieties is therefore the most effective measure to control this disease. However, conventional breeding approach for disease resistance is difficult, labor and cost time. The objective of this thesis is to research on bacterial wilt disease resistance of some tomato varieties through artificial infection and using marker SSR, so that we can identify resistant varieties to Ralstonia solanacearum isolate that collected in Vietnam.

The result of phenotypic evaluation by artificial infection methods showed that 7 bacterial isolates separated of two ecological Ho Chi Minh city and Lam Dong province, 3 races were exhibited the virulence. Result also showed Seeda was susceptible, Vimina 1 and Vimina 2 were resistants and F1 Safina 404, F1 Red Crown 250, F1 Mongal and F1 TN 323 were medium resistance. The result of PCR reaction showed that markers SSR306, SSR310 were located on chromosome 4, linked to Bw4 resistant gene, have polymorphism with 6 line of tomatoes such as F1 Safina 404, F1 Red Crown 250, F1 Mongal, F1 TN 323, F1 TG 105 CTS 386, Vimina1 and Vimina 2. This result showeds that the first step can use markers SSR306, SSR310 indicator for the purpose of selecting, creating tomato varieties resistant to bacterial wilt in Vietnam.

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