Welcome To Website IAS

Hot news
Achievement

Independence Award

- First Rank - Second Rank - Third Rank

Labour Award

- First Rank - Second Rank -Third Rank

National Award

 - Study on food stuff for animal(2005)

 - Study on rice breeding for export and domestic consumption(2005)

VIFOTEC Award

- Hybrid Maize by Single Cross V2002 (2003)

- Tomato Grafting to Manage Ralstonia Disease(2005)

- Cassava variety KM140(2010)

Centres
Website links
Vietnamese calendar
Library
Visitors summary
 Curently online :  3
 Total visitors :  4127218

Arabidopsis H+-ATPase AHA1 controls slow wave potential duration and wound-response jasmonate pathway activation
Thursday, 2019/10/03 | 08:01:16

Archana Kumari, Aurore Chételat, Chi Tam Nguyen, and Edward E. Farmer

 PNAS October 1, 2019 116 (40) 20226-20231

 Significance

Phosphorylation (P)-type ATPases act to maintain and modulate charge distribution across membranes and these proteins have been coopted for electrical signaling in animals. When wounded, membranes in the leaves of Arabidopsis thaliana depolarize rapidly. This is followed by a slower repolarization phase. We found that the proton pump AHA1 acted to control membrane potential when these plants were wounded. Specifically, the repolarization phase in aha1 mutants is prolonged relative to that in wild-type plants. In parallel, the jasmonate defense pathway is activated strongly and the mutant plants are better defended against herbivores than the wild type. We reveal that plants, like animals, use P-type ATPases in electrical signaling and show that AHA1 couples membrane potential to anti-herbivore defense.

Abstract

Electrogenic proton pumps have been implicated in the generation of slow wave potentials (SWPs), damage-induced membrane depolarizations that activate the jasmonate (JA) defense pathway in leaves distal to wounds. However, no defined H+-ATPases have been shown to modulate these electrical signals. Pilot experiments revealed that the proton pump activator fusicoccin attenuated SWP duration in Arabidopsis. Using mutant analyses, we identified Arabidopsis H+-ATPase 1 (AHA1) as a SWP regulator. The duration of the repolarization phase was strongly extended in reduced function aha1 mutants. Moreover, the duration of SWP repolarization was shortened in the presence of a gain-of-function AHA1 allele. We employed aphid electrodes to probe the effects of the aha1 mutation on wound-stimulated electrical activity in the phloem. Relative to the wild type, the aha1-7 mutant increased the duration and reduced the amplitudes of electrical signals in sieve tube cells. In addition to affecting electrical signaling, expression of the JA pathway marker gene JAZ10 in leaves distal to wounds was enhanced in aha1-7. Consistent with this, levels of wound-response jasmonoyl-isoleucine were enhanced in the mutant, as was defense against a lepidopteran herbivore. The work identifies a discrete member of the P-type ATPase superfamily with a role in leaf-to-leaf electrical signaling and plant defense.

 

See https://www.pnas.org/content/116/40/20226

 

Figure 1: Phenotype and wound-activated surface potential measurements in aha1-7, complemented aha1-7, and ost2-2D. (A) Five weeks-old wild-type (WT) rosette, +/aha1-7, and aha1-7/ aha1-7 rosettes. (B) Surface potential changes on leaf 8. Broken columns indicate measurements after wounding where repolarization taking more than 300 s was not quantified. (C) Surface potentials on distal leaf 13. (D) Five weeks-old WT, aha1-7, and aha1-7 complemented with AHA1p::AHA1-Venus. (E) Wound-activated surface potential changes on leaf 8, and (F) on distal leaf 13 (n = 10–12). (G and H) Wound-activated surface potential changes in distal leaf 13 of ost2-2D. (G) Amplitude and (H) duration, (n = 15). Data shown are means ± SD. Asterisks indicate a significant difference with WT. Student t test: *P < 0.05, **P < 0.01.


Back      Print      View: 16

[ Other News ]___________________________________________________
  • Genome-wide analysis of autophagy-associated genes in foxtail millet (Setaria italica L.) and characterization of the function of SiATG8a in conferring tolerance to nitrogen starvation in rice.
  • Arabidopsis small nucleolar RNA monitors the efficient pre-rRNA processing during ribosome biogenesis
  • XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves.
  • Reducing the Use of Pesticides with Site-Specific Application: The Chemical Control of Rhizoctonia solani as a Case of Study for the Management of Soil-Borne Diseases
  • OsJRL, a rice jacalin-related mannose-binding lectin gene, enhances Escherichia coli viability under high-salinity stress and improves salinity tolerance of rice.
  • Production of lipopeptide biosurfactants by Bacillus atrophaeus 5-2a and their potential use in microbial enhanced oil recovery.
  • GhABF2, a bZIP transcription factor, confers drought and salinity tolerance in cotton (Gossypium hirsutum L.).
  • Resilience of cassava (Manihot esculenta Crantz) to salinity: implications for food security in low-lying regions.
  • Cellulose synthase complexes act in a concerted fashion to synthesize highly aggregated cellulose in secondary cell walls of plants
  • No adverse effects of transgenic maize on population dynamics of endophytic Bacillus subtilis strain B916-gfp
  • Identification and expression analysis of OsLPR family revealed the potential roles of OsLPR3 and 5 in maintaining phosphate homeostasis in rice
  • Functional analysis of molecular interactions in synthetic auxin response circuits
  • Titanium dioxide nanoparticles strongly impact soil microbial function by affecting archaeal nitrifiers.
  • Inducible Expression of the De-Novo Designed Antimicrobial Peptide SP1-1 in Tomato Confers Resistance to Xanthomonas campestris pv. vesicatoria.
  • Toward combined delignification and saccharification of wheat straw by a laccase-containing designer cellulosome
  • SNP-based discovery of salinity-tolerant QTLs in a bi-parental population of rice (Oryza sativa)
  • Pinpointing genes underlying the quantitative trait loci for root-knot nematode resistance in palaeopolyploid soybean by whole genome resequencing.
  • Transcriptome- Assisted Label-Free Quantitative Proteomics Analysis Reveals Novel Insights into Piper nigrum -Phytophthora capsici Phytopathosystem.
  • Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants
  • Rapid hyperosmotic-induced Ca2+ responses in Arabidopsis thaliana exhibit sensory potentiation and involvement of plastidial KEA transporters
Designed & Powered by WEBSO CO.,LTD