Welcome To Website IAS

Hot news
Achievement

Independence Award

- First Rank - Second Rank - Third Rank

Labour Award

- First Rank - Second Rank -Third Rank

National Award

 - Study on food stuff for animal(2005)

 - Study on rice breeding for export and domestic consumption(2005)

VIFOTEC Award

- Hybrid Maize by Single Cross V2002 (2003)

- Tomato Grafting to Manage Ralstonia Disease(2005)

- Cassava variety KM140(2010)

Centres
Website links
Vietnamese calendar
Library
Visitors summary
 Curently online :  3
 Total visitors :  4153600

Artificial selection on GmOLEO1 contributes to the increase in seed oil during soybean domestication.
Wednesday, 2019/07/17 | 08:18:30

Zhang DZhang HHu ZChu SYu KLv LYang YZhang XChen XKan GTang YAn YCYu D.

PLoS Genet. 2019 Jul 10;15(7):e1008267. doi: 10.1371/journal.pgen.1008267. [Epub ahead of print]

Abstract

Increasing seed oil content is one of the most important breeding goals for soybean due to a high global demand for edible vegetable oil. However, genetic improvement of seed oil content has been difficult in soybean because of the complexity of oil metabolism. Determining the major variants and molecular mechanisms conferring oil accumulation is critical for substantial oil enhancement in soybean and other oilseed crops. In this study, we evaluated the seed oil contents of 219 diverse soybean accessions across six different environments and dissected the underlying mechanism using a high-resolution genome-wide association study (GWAS). An environmentally stable quantitative trait locus (QTL), GqOil20, significantly associated with oil content was identified, accounting for 23.70% of the total phenotypic variance of seed oil across multiple environments. Haplotype and expression analyses indicate that an oleosin protein-encoding gene (GmOLEO1), colocated with a leading single nucleotide polymorphism (SNP) from the GWAS, was significantly correlated with seed oil content. GmOLEO1 is predominantly expressed during seed maturation, and GmOLEO1 is localized to accumulated oil bodies (OBs) in maturing seeds. Overexpression of GmOLEO1 significantly enriched smaller OBs and increased seed oil content by 10.6% compared with those of control seeds. A time-course transcriptomics analysis between transgenic and control soybeans indicated that GmOLEO1 positively enhanced oil accumulation by affecting triacylglycerol metabolism. Our results also showed that strong artificial selection had occurred in the promoter region of GmOLEO1, which resulted in its high expression in cultivated soybean relative to wild soybean, leading to increased seed oil accumulation. The GmOLEO1 locus may serve as a direct target for both genetic engineering and selection for soybean oil improvement.

 

See https://www.ncbi.nlm.nih.gov/pubmed/31291251

Fig 1. GWAS for oil content in soybean seeds and candidate gene selection analyses.

A, A Manhattan plot for the BLUP of soybean oil content across six environments by association mapping. A red horizontal line depicts the Bonferroni-adjusted significance threshold (P<4.95×10−6). The x-axis shows the 20 soybean chromosomes, and the y-axis shows the significance expressed as the -log10P value. B, A zoomed-in Manhattan plot of the 0.2-Mb genomic region on either side of the most significant SNP at the QTL GqOil20 on chromosome 20. The red solid triangle represents the leading SNP (AX-93910018). The color intensity of other SNPs is shown according to their LDs (r2 value) with the leading SNP. Gene models within the region are indicated with blue rectangles, and the red rectangle represents the candidate gene Glyma.20G196600. The 50-kb genomic regions on both sides of the leading SNP are highlighted in light yellow. C, The extent of linkage disequilibrium (LD) in the 0.2-Mb genomic region on either side of the leading SNP based on pairwise r2 values. The r2 values are indicated using the color intensity index. D, Comparisons of seed oil content (%) between cultivated and wild soybeans. E, Comparison of GmOLEO1 expression between cultivated and wild soybeans. F, haplotypes of GmOLEO1 among 38 soybean genotypes. The orange and cyan rectangles on the promoter region indicate the cis-acting regulatory elements involved in ABA response and seed-specific regulation, respectively. G, Comparative analyses of the GmOLEO1 expression and oil content between the six different haplotypes. H, A neighbor-joining tree of the 38 accessions using variants from GmOLEO1. The edge color pattern was the same as that indicated by the six different haplotypes in (G). Black solid dots represent Gsoja accessions. I, Ratios of LUC and REN activity in Arabidopsis protoplasts transformed with recombinant plasmids containing the GmOLEO1 promoters from two different haplotypes (Hap1_pro, Hap5_pro) and the control vector. Significance analysis was performed using Fisher’s protected least significant difference (LSD) test. ** indicates a significant difference at the 0.01 level.

Back      Print      View: 89

[ Other News ]___________________________________________________
  • Genome-wide analysis of autophagy-associated genes in foxtail millet (Setaria italica L.) and characterization of the function of SiATG8a in conferring tolerance to nitrogen starvation in rice.
  • Arabidopsis small nucleolar RNA monitors the efficient pre-rRNA processing during ribosome biogenesis
  • XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves.
  • Reducing the Use of Pesticides with Site-Specific Application: The Chemical Control of Rhizoctonia solani as a Case of Study for the Management of Soil-Borne Diseases
  • OsJRL, a rice jacalin-related mannose-binding lectin gene, enhances Escherichia coli viability under high-salinity stress and improves salinity tolerance of rice.
  • Production of lipopeptide biosurfactants by Bacillus atrophaeus 5-2a and their potential use in microbial enhanced oil recovery.
  • GhABF2, a bZIP transcription factor, confers drought and salinity tolerance in cotton (Gossypium hirsutum L.).
  • Resilience of cassava (Manihot esculenta Crantz) to salinity: implications for food security in low-lying regions.
  • Cellulose synthase complexes act in a concerted fashion to synthesize highly aggregated cellulose in secondary cell walls of plants
  • No adverse effects of transgenic maize on population dynamics of endophytic Bacillus subtilis strain B916-gfp
  • Identification and expression analysis of OsLPR family revealed the potential roles of OsLPR3 and 5 in maintaining phosphate homeostasis in rice
  • Functional analysis of molecular interactions in synthetic auxin response circuits
  • Titanium dioxide nanoparticles strongly impact soil microbial function by affecting archaeal nitrifiers.
  • Inducible Expression of the De-Novo Designed Antimicrobial Peptide SP1-1 in Tomato Confers Resistance to Xanthomonas campestris pv. vesicatoria.
  • Toward combined delignification and saccharification of wheat straw by a laccase-containing designer cellulosome
  • SNP-based discovery of salinity-tolerant QTLs in a bi-parental population of rice (Oryza sativa)
  • Pinpointing genes underlying the quantitative trait loci for root-knot nematode resistance in palaeopolyploid soybean by whole genome resequencing.
  • Transcriptome- Assisted Label-Free Quantitative Proteomics Analysis Reveals Novel Insights into Piper nigrum -Phytophthora capsici Phytopathosystem.
  • Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants
  • Rapid hyperosmotic-induced Ca2+ responses in Arabidopsis thaliana exhibit sensory potentiation and involvement of plastidial KEA transporters
Designed & Powered by WEBSO CO.,LTD