Welcome To Website IAS

Hot news
Achievement

Independence Award

- First Rank - Second Rank - Third Rank

Labour Award

- First Rank - Second Rank -Third Rank

National Award

 - Study on food stuff for animal(2005)

 - Study on rice breeding for export and domestic consumption(2005)

VIFOTEC Award

- Hybrid Maize by Single Cross V2002 (2003)

- Tomato Grafting to Manage Ralstonia Disease(2005)

- Cassava variety KM140(2010)

Centres
Website links
Vietnamese calendar
Library
Visitors summary
 Curently online :  4
 Total visitors :  4099644

Bacillus subtilis biofilm induction by plant polysaccharides
Friday, 2013/04/26 | 08:24:09

Pascale B. Beauregarda, Yunrong Chaib,1, Hera Vlamakisa, Richard Losickb, and Roberto Koltera,2

 

Author Affiliations

 

1.aDepartment of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115; and
2.bDepartment of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138
  1. Edited by Sharon R. Long, Stanford University, Stanford, CA, and approved March 14, 2013 (received for review October 31, 2012)

 

Significance

 

The plant growth-promoting bacterium Bacillus subtilis is frequently found associated with plant roots where it protects plants from infection. Here, we demonstrate that B. subtilis root attachment depends on production of an extracellular matrix that holds the cells together in multicellular communities termed biofilms. We found that plant polysaccharides (major components of the plant’s cell wall) act as an environmental cue that triggers biofilm formation by the bacterium. Furthermore, these plant polysaccharides can serve as a carbon source used to produce the extracellular matrix. This work sheds light on how plants stimulate their colonization by this plant growth-promoting rhizobacterium.

 

Abstract

 

Bacillus subtilis is a plant-beneficial Gram-positive bacterium widely used as a biofertilizer. However, relatively little is known regarding the molecular processes underlying this bacterium's ability to colonize roots. In contrast, much is known about how this bacterium forms matrix-enclosed multicellular communities (biofilms) in vitro. Here, we show that, when B. subtilis colonizes Arabidopsis thaliana roots it forms biofilms that depend on the same matrix genes required in vitro. B. subtilis biofilm formation was triggered by certain plant polysaccharides. These polysaccharides served as a signal for biofilm formation transduced via the kinases controlling the phosphorylation state of the master regulator Spo0A. In addition, plant polysaccharides are used as a source of sugars for the synthesis of the matrix exopolysaccharide. The bacterium's response to plant polysaccharides was observed across several different strains of the species, some of which are known to have beneficial effects on plants. These observations provide evidence that biofilm genes are crucial for Arabidopsis root colonization by B. subtilis and provide insights into how matrix synthesis may be triggered by this plant.

 

http://www.pnas.org/content/110/17/E1621.abstract.html?etoc

PNAS April 23, 2013 vol. 110 no. 17 E1621-E1630

 

Fig. 1. B. subtilis cells colonizing A. thaliana roots expressmatrix genes. Wild-type (3610) cells harboring PtapA-yfp were coincubated with 6-d-old seedlings of A. thaliana and imaged at various time points postinoculation. Shown are overlays of fluorescence (false-colored green) and transmitted light (gray) images. Pictures are representative of at least ten independent roots. Arrows point toward some of the nonfluorescent cells. (Scale bars: 10μm.)

 

Back      Print      View: 1419

[ Other News ]___________________________________________________
  • Transgenic pig carrying green fluorescent proteasomes
  • Ammonia oxidation pathways and nitrifier denitrification are significant sources of N2O and NO under low oxygen availability
  • Genetic analysis and mapping of genes for resistance to multiple strains of Soybean mosaic virus in a single resistant soybean accession PI 96983
  • Genomic assay reveals tolerance of DNA damage by both translesion DNA synthesis and homology-dependent repair in mammalian cells
  • IRRI Scientists Develop Super Salt-tolerant Rice
  • A dominant major locus in chromosome 9 of rice (Oryza sativa L.) confers tolerance to 48°C high temperature at seedling stage.
  • Source-sink dynamics and proteomic reprogramming under elevated night temperature and their impact on rice yield and grain quality
  • Gene similarity networks provide tools for understanding eukaryote origins and evolution
  • Suppression of Arabidopsis protophloem differentiation and root meristem growth by CLE45 requires the receptor-like kinase BAM3
  • Scientists Re-evaluate Nutrient Uptake of Modern Corn
  • A Dominant Major Locus in Chromosome 9 of Rice (Oryza sativa L.) Confers Tolerance to 48 °C High Temperature at Seedling Stage
  • Scientists Identify Proton Pathway in Photosynthesis
  • Scientists Find Way to Increase Phosphorus Content in Wheat
  • De novo next-generation sequencing, assembling and annotation of Arachis hypogaea L. Spanish botanical type whole plant transcriptome
  • Targeted linkage map densification to improve cell wall related QTL detection and interpretation in maize
  • Fine-mapping and molecular marker development for Pi56(t), a NBS-LRR gene conferring broad-spectrum resistance to Magnaporthe oryzae in rice
  • Genome-wide comparative diversity uncovers multiple targets of selection for improvement in hexaploid wheat landraces and cultivars
  • Smoke-derived karrikin perception by the α/β-hydrolase KAI2 from Arabidopsis
  • USDA Approves Testing of Pink Pineapple
  • Characterization and fine mapping of the rice premature senescence mutant ospse1
Designed & Powered by WEBSO CO.,LTD