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Gene Editing Cockroaches with CRISPR-Cas9
Wednesday, 2022/05/25 | 06:25:54

Kyoto University and Institute of Evolutionary Biology (CSIC-Universitat Pompeu Fabra) scientists presented a simple and efficient technique for gene editing in insects. Their study is detailed in Cell Reports Methods.

 

Insects provide diversity and limitless possibilities for research tools that can help provide answers to fundamental questions about life. Gene editing of insects usually requires microinjection of materials into embryos, which is challenging in most species. Thus, the scientists established and optimized a new technique called “direct parental” CRISPR (DIPA-CRISPR), which entails adult injection and can be readily applied in any laboratory.

 

The researchers successfully tested DIPA-CRISPR to edit the eye color of cockroaches, which was not possible using conventional approaches. They also tested the technique in red flour beetle.  Because of its simplicity and accessibility, DIPA-CRISPR offers wider application in gene editing of insects.

 

Read more findings in Cell Reports Methods.

 

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