Welcome To Website IAS

Hot news

Independence Award

- First Rank - Second Rank - Third Rank

Labour Award

- First Rank - Second Rank -Third Rank

National Award

 - Study on food stuff for animal(2005)

 - Study on rice breeding for export and domestic consumption(2005)


- Hybrid Maize by Single Cross V2002 (2003)

- Tomato Grafting to Manage Ralstonia Disease(2005)

- Cassava variety KM140(2010)

Website links
Vietnamese calendar
Visitors summary
 Curently online :  7
 Total visitors :  5629075

I-SceI and customized meganucleases-mediated genome editing in tomato and oilseed rape
Tuesday, 2021/11/16 | 08:22:10

Benoit DaniloÉmilie MontesHéloïse ArchambeauMaryse LodéMathieu Rousseau-GueutinAnne-Marie Chèvre & Marianne Mazier

Transgenic Research (2021); on-line Published Oct. 11, 2021


Meganucleases are rare cutting enzymes that can generate DNA modifications and are part of the plant genome editing toolkit although they lack versatility. Here, we evaluated the use of two meganucleases, I-SceI and a customized meganuclease, in tomato and oilseed rape. Different strategies were explored for the use of these meganucleases. The activity of a customized and a I-SceI meganucleases was first estimated by the use of a reporter construct GFFP with the target sequences and enabled to demonstrate that both meganucleases can generate double-strand break and HDR mediated recombination in a reporter gene. Interestingly, I-SceI seems to have a higher DSB efficiency than the customized meganuclease: up to 62.5% in tomato and 44.8% in oilseed rape. Secondly, the same exogenous landing pad was introduced in both species. Despite being less efficient compared to I-SceI, the customized meganuclease was able to generate the excision of an exogenous transgene (large deletion of up to 3316 bp) present in tomato. In this paper, we also present some pitfalls to be considered before using meganucleases (e.g., potential toxicity) for plant genome editing.


See: https://link.springer.com/article/10.1007/s11248-021-00287-2

Back      Print      View: 54

[ Other News ]___________________________________________________
  • Auxin depletion from leaf primordia contributes to organ patterning
  • Phytochrome controls alternative splicing to mediate light responses in Arabidopsis
  • Maternal temperature history activates Flowering Locus T in fruits to control progeny dormancy according to time of year
  • Identification and evaluation of quantitative trait loci underlying resistance to multiple HG types of soybean cyst nematode in soybean PI 437655
  • Factor analytic mixed models for the provision of grower information from national crop variety testing programs
  • Exploring the areas of applicability of whole genome prediction methods for Asian rice (Oryza sativa L.)
  • Investigation of terpene diversification across multiple sequenced plant genomes
  • Arabidopsis ribosomal proteins control vacuole trafficking and developmental programs through the regulation of lipid metabolism
  • Effect of the ahas Transgene on Biological Nitrogen Fixation and Yield of Soybean
  • Arabidopsis ROCK1 transports UDP-GlcNAc/UDP-GalNAc and regulates ER protein quality control and cytokinin activity
  • A peripheral endocannabinoid mechanism contributes to glucocorticoid-mediated metabolic syndrome
  • High-Resolution Linkage Map and Chromosome-Scale Genome Assembly for Cassava (Manihot esculenta Crantz) from 10 Populations
  • Analysis of the Transcriptome of Banana Fruit during Ripening
  • Nitric oxide negatively regulates abscisic acid signaling in guard cells by S-nitrosylation of OST1
  • Salt Tolerant Gene in Soybean Identified
  • Climate change decouples oceanic primary and export productivity and organic carbon burial
  • Evolution of the H9N2 influenza genotype that facilitated the genesis of the novel H7N9 virus
  • 5-Hydroxymethylcytosine Is Not Present in Appreciable Quantities in Arabidopsis DNA
  • Fine mapping of the qLOP2 and qPSR2 1 loci associated with chilling stress tolerance of wild rice seedlings
  • Natural diversity in daily rhythms of gene expression contributes to phenotypic variation


Designed & Powered by WEBSO CO.,LTD