Welcome To Website IAS

Hot news

Independence Award

- First Rank - Second Rank - Third Rank

Labour Award

- First Rank - Second Rank -Third Rank

National Award

 - Study on food stuff for animal(2005)

 - Study on rice breeding for export and domestic consumption(2005)


- Hybrid Maize by Single Cross V2002 (2003)

- Tomato Grafting to Manage Ralstonia Disease(2005)

- Cassava variety KM140(2010)

Website links
Vietnamese calendar
Visitors summary
 Curently online :  4
 Total visitors :  5448955

Metabolic engineering of astaxanthin-rich maize and its use in the production of biofortified eggs
Monday, 2021/09/20 | 09:22:23

Xiaoqing Liu, Xuhui Ma, Hao Wang, Suzhen Li, Wenzhu Yang, Ramdhan Dwi Nugroho, Lili Luo, Xiaojin Zhou, Chaohua Tang, Yunliu Fan, Qingyu Zhao, Junmin Zhang, Rumei Chen.

Plant Biotechnology Journal; 29 March 2021; https://doi.org/10.1111/pbi.13593


Production of the high-value carotenoid astaxanthin, which is widely used in food and feed due to its strong antioxidant activity and colour, is less efficient in cereals than in model plants. Here, we report a new strategy for expressing β-carotene ketolase and hydroxylase genes from algae, yeasts and flowering plants in the whole seed using a seed-specific bidirectional promoter. Engineered maize events were backcrossed to inbred maize lines with yellow endosperm to generate progenies that accumulate astaxanthin from 47.76 to 111.82 mg/kg DW in seeds, and the maximum level is approximately sixfold higher than those in previous reports (16.2–16.8 mg/kg DW) in cereals. A feeding trial with laying hens indicated that they could take up astaxanthin from the maize and accumulate it in egg yolks (12.10–14.15 mg/kg) without affecting egg production and quality, as observed using astaxanthin from Haematococcus pluvialis. Storage stability evaluation analysis showed that the optimal conditions for long-term storage of astaxanthin-rich maize are at 4 °C in the dark. This study shows that co-expressing of functional genes driven by seed-specific bidirectional promoter could dramatically boost astaxanthin biosynthesis in every parts of kernel including embryo, aleurone layer and starch endosperm other than previous reports in the starch endosperm only. And the staple crop maize could serve as a cost-effective plant factory for reliably producing astaxanthin.


See: https://onlinelibrary.wiley.com/doi/10.1111/pbi.13593


Figure 2: Development of astaxanthin-rich maize. (a) Schematic diagram of the constructs pBDEN-CP-BZ and pBDEN-CP-BZ-RNAi, functional genes and sense (lcy-e) and anti-sense (lcy-e′) fragments of ZmLCY-e directed by the seed-specific bidirectional promoter PR5SGPA. (b–g) Engineered astaxanthin-rich maize seeds at different developmental stages; (b–d) seeds derived from pBDEN-CP-BZ; (e–g) seeds derived from pBDEN-CP-BZ-RNAi; (b, e) at 20 days after pollination (DAP); (c, f) at 30 DAP; (d, g) mature dry seeds. (h–l) High-performance liquid chromatography (HPLC) analysis of astaxanthin in dried astaxanthin-rich maize seeds; (h) a mixture of all-E, 9Z and 13Z astaxanthin standards; (i) the transgenic event LX68-1, derived from pBDEN-CP-BZ; (j) the transgenic event LX71-2, derived from pBDEN-CP-BZ-RNAi; (k) the yellow endosperm inbred line Z58 used as the recurrent parent; (l) quantitative analysis of isomers and total astaxanthin. all-E, all-trans geometric isomer; 9Z, cis-9 geometric isomer; 13Z, cis-13 geometric isomer. All data were derived from three technical repetitions.

Back      Print      View: 39

[ Other News ]___________________________________________________
  • Genome-wide analysis of autophagy-associated genes in foxtail millet (Setaria italica L.) and characterization of the function of SiATG8a in conferring tolerance to nitrogen starvation in rice.
  • Arabidopsis small nucleolar RNA monitors the efficient pre-rRNA processing during ribosome biogenesis
  • XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves.
  • Reducing the Use of Pesticides with Site-Specific Application: The Chemical Control of Rhizoctonia solani as a Case of Study for the Management of Soil-Borne Diseases
  • OsJRL, a rice jacalin-related mannose-binding lectin gene, enhances Escherichia coli viability under high-salinity stress and improves salinity tolerance of rice.
  • Production of lipopeptide biosurfactants by Bacillus atrophaeus 5-2a and their potential use in microbial enhanced oil recovery.
  • GhABF2, a bZIP transcription factor, confers drought and salinity tolerance in cotton (Gossypium hirsutum L.).
  • Resilience of cassava (Manihot esculenta Crantz) to salinity: implications for food security in low-lying regions.
  • Cellulose synthase complexes act in a concerted fashion to synthesize highly aggregated cellulose in secondary cell walls of plants
  • No adverse effects of transgenic maize on population dynamics of endophytic Bacillus subtilis strain B916-gfp
  • Identification and expression analysis of OsLPR family revealed the potential roles of OsLPR3 and 5 in maintaining phosphate homeostasis in rice
  • Functional analysis of molecular interactions in synthetic auxin response circuits
  • Titanium dioxide nanoparticles strongly impact soil microbial function by affecting archaeal nitrifiers.
  • Inducible Expression of the De-Novo Designed Antimicrobial Peptide SP1-1 in Tomato Confers Resistance to Xanthomonas campestris pv. vesicatoria.
  • Toward combined delignification and saccharification of wheat straw by a laccase-containing designer cellulosome
  • SNP-based discovery of salinity-tolerant QTLs in a bi-parental population of rice (Oryza sativa)
  • Pinpointing genes underlying the quantitative trait loci for root-knot nematode resistance in palaeopolyploid soybean by whole genome resequencing.
  • Transcriptome- Assisted Label-Free Quantitative Proteomics Analysis Reveals Novel Insights into Piper nigrum -Phytophthora capsici Phytopathosystem.
  • Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants
  • Rapid hyperosmotic-induced Ca2+ responses in Arabidopsis thaliana exhibit sensory potentiation and involvement of plastidial KEA transporters


Designed & Powered by WEBSO CO.,LTD