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Mutations in sorghum SBEIIb and SSIIa affect alkali spreading value, starch composition, thermal properties and flour viscosity
Saturday, 2019/12/07 | 05:32:33

Stefanie Griebel, Richard P. Westerman, Adedayo Adeyanju, Charles Addo-Quaye, Bruce A. Craig, Clifford F. Weil, Suzanne M. Cunningham, Bhavesh Patel, Osvaldo H. Campanella, Mitchell R. Tuinstra

Theoretical and Applied Genetics

December 2019, Volume 132, Issue 12pp 3357–3374

Key message

Seven novel alleles of SBEIIb and one allele of SSIIa co-segregated with the ASV phenotype and contributed to distinct starch quality traits important for food-processing applications.

Abstract

Sorghum is an important food crop for millions of people in Africa and Asia. Whole-genome re-sequencing of sorghum EMS mutants exhibiting an alkali spreading value (ASV) phenotype revealed candidate SNPs in Sobic.004G163700 and Sobic.010G093400. Comparative genomics identified Sobic.010G093400 as a starch synthase IIa and Sobic.004G163700 as a starch branching enzyme IIb. Segregation analyses showed that mutations in Sobic.010G093400 or Sobic.004G163700 co-segregated with the ASV phenotype. Mutants in SSIIa exhibited no change in amylose content but expressed lower final viscosity and lower starch gelatinization temperature (GT) than starches from non-mutant plants. The sbeIIb mutants exhibited significantly higher amylose levels and starch GT and lower viscosity compared to non-mutant starches and ssIIa mutants. Mutations in SBEIIb had a dosage-dependent effect on amylose content. Double mutants of sbeIIb and ssIIa resembled their sbeIIb parent in amylose content, starch thermal properties and viscosity profiles. These variants will provide opportunities to produce sorghum varieties with modified starch end-use qualities important for the beer brewing and baking industries and specialty foods for humans with diabetes.

See https://link.springer.com/article/10.1007/s00122-019-03430-0

 

 

Figure 1: Mutations in SBEIIb and SSIIa

a mutants and positions of SNPs in sbeIIb (Sobic.004G163700), 

bSBEIIb mutations, effects and impacts predicted by snpEff on transcription and translation, 

c mutants and positions of SNPs in ssIIa (Sobic.010G093400),

and d ssIIa mutations, effects and impacts predicted by snpEff on transcription and translation.

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