Welcome To Website IAS

Hot news
Achievement

Independence Award

- First Rank - Second Rank - Third Rank

Labour Award

- First Rank - Second Rank -Third Rank

National Award

 - Study on food stuff for animal(2005)

 - Study on rice breeding for export and domestic consumption(2005)

VIFOTEC Award

- Hybrid Maize by Single Cross V2002 (2003)

- Tomato Grafting to Manage Ralstonia Disease(2005)

- Cassava variety KM140(2010)

Centres
Website links
Vietnamese calendar
Library
Visitors summary
 Curently online :  13
 Total visitors :  4972321

Natural variation in OsGASR7 regulates grain length in rice
Monday, 2021/01/18 | 08:23:09
 

 

Zhengbin TangXiuying GaoXiangyun ZhanNengyan FangRuqin WangChengfang ZhanJiaqi ZhangGuang CaiJinping ChengYongmei BaoHongsheng ZhangJi Huang

Plant Biotechnology Journal; 2021 Jan;19(1):14-16.  doi: 10.1111/pbi.13436.  

Identification of quantitative trait loci (QTLs) for grain length (GL) is important to rice breeding for increasing grain yield and appearance quality. Recent studies have identified a number of QTLs/genes as key grain length regulators by linkage mapping or genome‐wide association study (GWAS). These regulators are involved in G protein signalling, phytohormone signalling or transcriptional regulation, etc (Li et al., 2018). However, our current knowledge on GL is still fragmented in molecular mechanism and breeding utilization in rice. Here, we detected QTLs for GL by GWAS using 210 rice accessions from rice diversity panel 1 (RDP1) (McCouch et al., 2016) and linkage mapping using a recombinant inbred line (RIL) population with 116 lines derived from geng/japonica rice Suyunuo (long grain) and Bodao (short grain) (Figure 1a). Interestingly, one co‐localized locus was identified and LOC_Os06g15620 encoding a gibberellic acid‐stimulated regulator (GASR) protein included in this region was confirmed to control GL.

 

See https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769224/

Figure 1:

Cloning and variation analysis of OsGASR7 for grain length in rice. (a) Grain morphology of Bodao (BD), Suyunuo (SYN) and some representative lines/accessions from RILs/RDP1. Scale bar, 5 mm. (b) Identification and positional cloning of OsGASR7 combining GWAS and linkage mapping. (b1) The genome‐wide association signals for grain length are shown in the region at about 8.5–10.0 Mb on chromosome 6 (x‐axis). Negative log10‐transformed P‐values from the EMMAX algorithm are plotted on the y‐axis. The position of the peak SNP is indicated by the red dot. The horizontal red line indicates the threshold (−log10(P) = 5.6). (b2) The QTL for grain length is shown in the region at 8.848–8.91 Mb on chromosome 6 (x‐axis). LOD values from ICIM‐ADD algorithm are plotted on the y‐axis. The horizontal red line is the LOD threshold (2.82). (b3) The physical position of the predicted ORFs (filled arrows). (b4) Non‐synonymous variants of OsGASR7 between Bodao and Suyunuo. Black lines represent introns, and colour bars represent exons. (c) OsGASR7 controls grain length. (c1, c2) Grain morphology of wild‐type Bodao (BD), Nipponbare (Nip) and transgenic complemental rice BD‐pOsGASR7SYN::OsGASR7SYN (B_CP), Nip‐pOsGASR7SYN::OsGASR7SYN (N_CP). Scale bar, 5 mm. (c3, c4) Statistical analysis of (brown) grain length of wild‐type plants and transgenic plants. The dashed lines and error lines represent means ± SD (n ≥ 4 plants, shown by dots). (c5) SEM observation of mature seeds. Scale bar, 1 mm for seed and 100 μm for lemma. (c6) Statistical analysis of cell length of the glume outer surfaces. Data are presented as means ± SD (n = 9). The P‐values are calculated by Student's t‐test. (d) InDel1 is significantly associated with grain length. (d1) The x‐axis indicates position of each variation in the ORF of OsGASR7 and the y‐axis is negative log10‐transformed P‐values from GLM algorithm. (d2) Six major genotypes of OsGASR7. (e) Genotype analysis of OsGASR7 for subpopulation distribution and grain length in 3K accessions (e1) and cultivars (e2). GJgeng/japonicaXIxian/indicacAcentrumAuscBcentrumBasmatiBDOsGASR7BDSYNOsGASR7SYN; I: OsGASR7I;II: OsGASR7II; III: OsGASR7III; Ⅳ: OsGASR7nonSYN: cultivars without OsGASR7SYN; number in brackets: number of statistical samples. Significance analysis is performed by Student's t‐test. (f) The subcellular localization of OsGASR7BD‐GFP and OsGASR7SYN‐GFP. Scale bar, 5 μm.

Back      Print      View: 37

[ Other News ]___________________________________________________
  • Genome-wide analysis of autophagy-associated genes in foxtail millet (Setaria italica L.) and characterization of the function of SiATG8a in conferring tolerance to nitrogen starvation in rice.
  • Arabidopsis small nucleolar RNA monitors the efficient pre-rRNA processing during ribosome biogenesis
  • XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves.
  • Reducing the Use of Pesticides with Site-Specific Application: The Chemical Control of Rhizoctonia solani as a Case of Study for the Management of Soil-Borne Diseases
  • OsJRL, a rice jacalin-related mannose-binding lectin gene, enhances Escherichia coli viability under high-salinity stress and improves salinity tolerance of rice.
  • Production of lipopeptide biosurfactants by Bacillus atrophaeus 5-2a and their potential use in microbial enhanced oil recovery.
  • GhABF2, a bZIP transcription factor, confers drought and salinity tolerance in cotton (Gossypium hirsutum L.).
  • Resilience of cassava (Manihot esculenta Crantz) to salinity: implications for food security in low-lying regions.
  • Cellulose synthase complexes act in a concerted fashion to synthesize highly aggregated cellulose in secondary cell walls of plants
  • No adverse effects of transgenic maize on population dynamics of endophytic Bacillus subtilis strain B916-gfp
  • Identification and expression analysis of OsLPR family revealed the potential roles of OsLPR3 and 5 in maintaining phosphate homeostasis in rice
  • Functional analysis of molecular interactions in synthetic auxin response circuits
  • Titanium dioxide nanoparticles strongly impact soil microbial function by affecting archaeal nitrifiers.
  • Inducible Expression of the De-Novo Designed Antimicrobial Peptide SP1-1 in Tomato Confers Resistance to Xanthomonas campestris pv. vesicatoria.
  • Toward combined delignification and saccharification of wheat straw by a laccase-containing designer cellulosome
  • SNP-based discovery of salinity-tolerant QTLs in a bi-parental population of rice (Oryza sativa)
  • Pinpointing genes underlying the quantitative trait loci for root-knot nematode resistance in palaeopolyploid soybean by whole genome resequencing.
  • Transcriptome- Assisted Label-Free Quantitative Proteomics Analysis Reveals Novel Insights into Piper nigrum -Phytophthora capsici Phytopathosystem.
  • Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants
  • Rapid hyperosmotic-induced Ca2+ responses in Arabidopsis thaliana exhibit sensory potentiation and involvement of plastidial KEA transporters

 

Designed & Powered by WEBSO CO.,LTD