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Solation and characterization of a c-repeat binding factor gene from tevang-1 maize cultivar
Friday, 2019/10/04 | 08:22:22

Nguyen Thuy Linh, Luu Han Ly, Nguyen Thuy Duong, Huynh Thi Thu Hue

 Academia Journal of Biology 2019 41 (3):85-100

 ABSTRACT

 C-repeat binding factor (CBF) proteins are transcription factors involved in plant response to abiotic stresses, especially low-temperature condition. In this research, a CBF3-coding gene was isolated from a cold-acclimation maize variety, Zea mays var. Tevang-1 and denoted as ZmCBF3tv. The isolated gene shared 96.49% homology with the B73-reference gene and had no intron in the coding sequence. By using bioinformatic tools, a number of variations in the nucleotide and amino acid sequences were identified. An alignment between ZmCBF3tv and other CBF/DREB1 proteins from various species revealed functional regions and typical features, such as nuclear localization signal (NLS), the AP2 DNA-binding domain, and acidic-amino-acidrich segments. Additionally, a phylogenetic analysis based on the AP2 domain showed that the maize CBF3 transcription factor had the highest similarity with that from rice and closely related to other CBF/DREB1 protein of monocots. The function of the ZmCBF3tv product is suggested to be a CBF/DREB1 transcription factor. Keywords: CBF/DREB1, transcription factor, Tevang-1, maize, cold tolerance.

 

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Figure 1. Isolation of ZmCBF3tv gene from Tevang-1 maize: a. Result of total RNA extraction. 1–3: Total RNA from maize plants after 24, 48, and 72 hours under low-temperature condition, respectively; b. Result of RT-PCR reaction. M: GeneRuler 1 kb DNA Ladder (Thermo Scientific, USA). 1–3: PCR products using cDNA templates of 24, 48, and 72-hours cold-treated samples, respectively


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