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Stability of Transgene Inheritance in Progeny of Field-Grown Pear Trees over a 7-Year Period
Thursday, 2022/01/20 | 07:01:28

Vadim Lebedev

Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Prospekt Nauki 6, 142290 Pushchino, Moscow Region, Russia

Academic Editors: Radu E. Sestras, Jaime Prohens, Adriana F. Sestras and Mariola Plazas

Plants 202211(2), 151; Published: 6 January 2022

Abstract

Breeding woody plants is a very time-consuming process, and genetic engineering tools have been used to shorten the juvenile phase. In addition, transgenic trees for commercial cultivation can also be used in classical breeding, but the segregation of transgenes in the progeny of perennial plants, as well as the possible appearance of unintended changes, have been poorly investigated. We studied the inheritance of the uidA gene in the progeny of field-grown transgenic pear trees for 7 years and the physical and physiological parameters of transgenic seeds. A total of 13 transgenic lines were analyzed, and the uidA gene segregated 1:1 in the progeny of 9 lines and 3:1 in the progeny of 4 lines, which is consistent with Mendelian inheritance for one and two transgene loci, respectively. Rare and random deviations from the Mendelian ratio were observed only for lines with one locus. Transgenic seeds’ mass, size, and shape varied slightly, despite significant fluctuations in weather conditions during cultivation. Expression of the uidA gene in the progeny was stable. Our study showed that the transgene inheritance in the progeny of pear trees under field conditions occurs according to Mendelian ratio, does not depend on the environment, and the seed vigor of transgenic seeds does not change.

 

See https://www.mdpi.com/2223-7747/11/2/151/htm

 

Figure 5. Segregation of GUS activity in pear progeny: (a) non-transgenic control; (b) ratio 1:1; (c) ratio 3:1.

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