Welcome To Website IAS

Hot news
Achievement

Independence Award

- First Rank - Second Rank - Third Rank

Labour Award

- First Rank - Second Rank -Third Rank

National Award

 - Study on food stuff for animal(2005)

 - Study on rice breeding for export and domestic consumption(2005)

VIFOTEC Award

- Hybrid Maize by Single Cross V2002 (2003)

- Tomato Grafting to Manage Ralstonia Disease(2005)

- Cassava variety KM140(2010)

Centres
Website links
Vietnamese calendar
Library
Visitors summary
 Curently online :  11
 Total visitors :  7437600

The Application of CRISPR in Dendrobium Orchids
Saturday, 2016/12/31 | 05:56:05

Dendrobium officinale is a special orchid species that can grow without vernalization. Since the genome sequence of D. officinale is available, it could become a research model for studies of the family Orchidaceae. However, genetic manipulation in D. officinale is poorly studied. Hence, Chinese scientists, led by Ling Kui, aim to successfully apply the CRISPR/Cas9 system to manipulate D. officinale.

 

Using Agrobacterium-mediated transformation, the team applied the CRISPR/Cas9-mediated genome editing system in D. officinale by selecting five target genes (C3H, C4H, 4CL, CCR and IRX) in the lignocellulose biosynthesis pathway. Results show that the technique can generate edits at a rate of 10 to 100%. The team also compared gene activity under different promoters and found that MMV, CVMV, and PCISV were as effective as the 35S promoter used in the transformation.

 

These results show that genetic manipulation tools can efficiently express exogenous genes as well as edit genes in D. officinale. These tools can help create novel D. officinale varieties and may also advance the molecular investigation of the Orchidaceae family.

 

For more information on this study, read the article in Frontiers in Plant Science.

Back      Print      View: 642

[ Other News ]___________________________________________________
  • Beyond genes: Protein atlas scores nitrogen fixing duet
  • 2016 Borlaug CAST Communication Award Goes to Dr. Kevin Folta
  • FAO and NEPAD team up to boost rural youth employment in Benin, Cameroon, Malawi and Niger
  • Timely seed distributions in Ethiopia boost crop yields, strengthen communities’ resilience
  • Parliaments must work together in the final stretch against hunger
  • Empowering women farmers in the polder communities of Bangladesh
  • Depression: let’s talk
  • As APEC Concludes, CIP’s Food Security and Climate Smart Agriculture on Full Display
  • CIAT directly engages with the European Cocoa Industry
  • Breeding tool plays a key role in program planning
  • FAO: Transform Agriculture to Address Global Challenges
  • Uganda Holds Banana Research Training for African Scientists and Biotechnology Regulators
  • US Congress Ratifies Historic Global Food Security Treaty
  • Fruit Fly`s Genetic Code Revealed
  • Seminar at EU Parliament Tackles GM Crops Concerns
  • JICA and IRRI ignites a “seed revolution” for African and Asian farmers
  • OsABCG26 Vital in Anther Cuticle and Pollen Exine Formation in Rice
  • Akira Tanaka, IRRI’s first physiologist, passes away
  • WHO calls for immediate safe evacuation of the sick and wounded from conflict areas
  • Farmer Field School in Tonga continues to break new ground in the Pacific for training young farmers

 

Designed & Powered by WEBSO CO.,LTD