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The thiol-disulfide exchange activity of AtPDI1 is involved in the response to abiotic stresses
Wednesday, 2022/01/19 | 07:50:56

Ying LuLi YuanZhou ZhouMengyu WangXiaoyun WangShizhong Zhang & Qinghua Sun

BMC Plant Biology volume 21, Article number: 557 (2021) Published: 23 November 2021

Background

Arabidopsis protein disulfide isomerase 1 (AtPDI1) has been demonstrated to have disulfide isomerase activity and to be involved in the stress response. However, whether the anti-stress function is directly related to the activities of thiol-disulfide exchange remains to be elucidated.

Results

In the present study, encoding sequences of AtPDI1 of wild-type (WT) and double-cysteine-mutants were transformed into an AtPDI1 knockdown Arabidopsis line (pdi), and homozygous transgenic plants named pdi-AtPDI1, pdi-AtPDI1m1 and pdi-AtPDI1m2 were obtained. Compared with the WT and pdi-AtPDI1, the respective germination ratios of pdi-AtPDI1m1 and pdi-AtPDI1m2 were significantly lower under abiotic stresses and exogenous ABA treatment, whereas the highest germination rate was obtained with AtPDI1 overexpression in the WT (WT- AtPDI1). The root length among different lines was consistent with the germination rate; a higher germination rate was observed with a longer root length. When seedlings were treated with salt, drought, cold and high temperature stresses, pdi-AtPDI1m1pdi-AtPDI1m2 and pdi displayed lower survival rates than WT and AtPDI1 overexpression plants. The transcriptional levels of ABA-responsive genes and genes encoding ROS-quenching enzymes were lower in pdi-AtPDI1m1 and pdi-AtPDI1m2 than in pdi-AtPDI1.

Conclusion

Taken together, these results clearly suggest that the anti-stress function of AtPDI1 is directly related to the activity of disulfide isomerase.

 

See: https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-021-03325-7

 

Fig.1: Seed germination of different AtPDI1 lines on 1/2 MS medium containing H2O2, NaCl or mannitol. A Phenotypes of different AtPDI1 lines (WT, pdi, WT-AtPDI1, pdi-AtPDI1, pdi-AtPDI1m1 and pdi-AtPDI1m2) under abiotic stress. B Gemination process according to radicle germination. C Gemination process according to cotyledon germination. Each experiment was carried out in three biological replicates

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