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UPenn Develops Lettuce That Stimulates Bone Building Cells
Sunday, 2020/03/22 | 06:36:19
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A team of scientists from the University of Pennsylvania's School of Dental Medicine developed lettuce plants that could stimulate the growth of bone-building cells and promote bone regeneration by using an animal protein to synthesize an orally delivered shelf-stable medication grown in the lettuce. This can address the medication needs of bone-fracture and diabetic patients who can have the option of eating their medicine rather than use injectables.
The scientists inserted the DNA of a protein called the Insulin-like Growth Factor 1 or IGF-1 into the lettuce genome. IGF-1 is an animal protein that can pass through the stomach and be absorbed in the gut. The protein is known to signal the increase in the number and longevity of the cells responsible for bone growth promoting fracture healing, and dental tissue regeneration. This is particularly important for older adults who are at risk for fracture, and also for diabetics who have low IGF-1 levels. Diabetic patients are prone to bone fractures more than the general public and are known to heal slower. Though there are existing treatments to address this, patients often skip them because they are expensive and require a repetitive process of injections.
The developed lettuce was grown in the laboratory and later on was put to the test by feeding it to diabetic mice with broken bones. Results showed that the mice had an increase of IGF-1 levels in their bloodstream and it stayed that way for a few hours. Further testing showed that the mice had a significant increase in bone volume and density at their fracture sites. The scientists also found that the lettuce leaves can be freeze-dried and ground into powder form and is shelf-stable for nearly three years.
This new development can become an option for diabetic patients with bone fractures. Instead of seeking treatment using repetitive injectables with a short shelf-life span that need to be refrigerated, it is possible to produce a plant-based solution, in this case, the lettuce, that can be eaten, stored and transported at room temperature, and can be grown, thus qualifying as a renewable resource of the medication.
The scientists published the results of their study in Biomaterials.
Figure: Generation of lettuce/tobacco transplastomic lines expressing codon-optimized CTB-Pro-IGF-1 gene. (A) Schematic illustration of CTB-Pro-IGF-1 expression cassette for lettuce chloroplast transformation. (A and E) 16s rRNA and 23s rRNA, 16s and 23s ribosomal RNA; trnI, isoleucyl-tRNA; trnA, alanyl-tRNA; Prrn, rRNA operon promoter; aadA, aminoglycoside 3′-adenylytransferase; TrbcL, 3′ UTR of ribulose bisphosphate carboxylase large subunit; PpsbA, promoter of psbA; CTB-Pro-IGF-1(co), codon optimized premature form of human insulin-like growth factor-1 with CTB (Cholera non-toxic B subunit) fusion; TpsbA, 3′-UTR of psbA; SB-P, Southern blot probe. Primer sets used for PCR screening are indicated in arrows. (B) Genomic DNA PCR screening, (C) Southern blot analysis, and (D) Protein expressions of the lettuce lines expressing CTB-Pro-IGF-1. Expected sizes are indicated in arrows (24.3 kDa). (B–D) Lanes 1 to 4, individual transplastomic lines; WT, untransformed wild type; LS, lettuce. (E) Schematic illustration of CTB-Pro-IGF-1 expression cassette for tobacco chloroplast transformation. (F) Southern blot analysis of CTB-Pro-IGF1 tobacco transplastomic lines. WT, untransformed wild type; lanes 1 and 2, transplastomic lines; PH, tobacco Petit Havana. |
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