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A Dominant Major Locus in Chromosome 9 of Rice (Oryza sativa L.) Confers Tolerance to 48 °C High Temperature at Seedling Stage

In an earlier greenhouse screening, we identified a local indica cultivar HT54 tolerant to high temperature at both seedling and grain-filling stages. In this study, we develop an optimized procedure for fine assessment of this heat tolerance.

Journal of Heredity 2013:104(2):287–294

He Wei,  Jianping Liu, Ya Wang, Nongrong Huang, Xiaobo ZHang, LiangcHao Wang, JiWen ZHang, Jumin Tu, andXuHua ZHong

Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Yuhangtang Road 388, Hangzhou

310058, China (Wei, Liu, Wang, Zhang, Wang, Zhang, and Tu); and Rice Research Institute, Guangdong Academy of

Agricultural Sciences, Dongyi Street No 3, Jinyin Road, Guanzhou 510640, China (Huang and Zhong).

 

Abstract

 

In an earlier greenhouse screening, we identified a local indica cultivar HT54 tolerant to high temperature at both seedling and grain-filling stages. In this study, we develop an optimized procedure for fine assessment of this heat tolerance. The results indicated that HT54 seedlings could tolerate high temperature up to 48 °C for 79 h. The genetic analysis of F1and F2 off-spring derived from the cross between HT54 and HT13, a heat-sensitive breeding line, reveals that the heat tolerance of HT54 was controlled by a dominant major locus, which has been designated as OsHTAS(Oryza sativa heat tolerance at seedling stage). This locus was mapped on rice chromosome 9 within an interval of 420 kb between markers of InDel5 and RM7364. The determined candidate ZFPgene has been confirmed to be cosegregated with a single nucleotide polymorphism (SNP)

 

developed PCR-restriction fragment length polymorphism (RFLP) marker RBsp1407 in its promoter region. Another heat tolerance-associated SNP was identified in the first intron of its 5′-untranslated region. The existence of these SNPs thereby  indicated that the OsHTASlocus contains at least two alleles. We named the one from HT54 as OsHTASa and the one from HT13 as OsHTASb. Further dynamic expression analysis demonstrated that OsHTASa was actively responsive to 45 °C high temperature stress compared with the OsHTASb allele.

 

Key words:  cosegregation, gene mapping, OsHTAS, PCR-RFLP, RBsp1407

 

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