Welcome To Website IAS

Hot news
Achievement

Independence Award

- First Rank - Second Rank - Third Rank

Labour Award

- First Rank - Second Rank -Third Rank

National Award

 - Study on food stuff for animal(2005)

 - Study on rice breeding for export and domestic consumption(2005)

VIFOTEC Award

- Hybrid Maize by Single Cross V2002 (2003)

- Tomato Grafting to Manage Ralstonia Disease(2005)

- Cassava variety KM140(2010)

Centres
Website links
Vietnamese calendar
Library
Visitors summary
 Curently online :  5
 Total visitors :  5436833

Engineering Resistance to Bacterial Blight and Bacterial Leaf Streak in Rice

Xanthomonas oryzae (Xo) is one of the important pathogenic bacterial groups affecting rice production. Its pathovars Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc) cause bacterial blight and bacterial leaf streak in rice, respectively. Xo infects host plants by relying mainly on its transcription activator-like effectors (TALEs) that bind to host DNA targets, named effector binding elements (EBEs), and induce the expression of downstream major susceptibility genes. Blocking TALE binding to EBE could increase rice resistance to the corresponding Xo.

Zhe NiYongqiang CaoXia JinZhuomin FuJianyuan LiXiuyu MoYongqiang HeJiliang TangSheng Huang

Rice (N Y); 2021 Apr 23;14(1):38.  doi: 10.1186/s12284-021-00482-z.

 

Abstract

 

Background: Xanthomonas oryzae (Xo) is one of the important pathogenic bacterial groups affecting rice production. Its pathovars Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc) cause bacterial blight and bacterial leaf streak in rice, respectively. Xo infects host plants by relying mainly on its transcription activator-like effectors (TALEs) that bind to host DNA targets, named effector binding elements (EBEs), and induce the expression of downstream major susceptibility genes. Blocking TALE binding to EBE could increase rice resistance to the corresponding Xo.

 

Findings: We used CRISPR/Cas9 to edit the EBEs of three major susceptibility genes (OsSWEET11, OsSWEET14 and OsSULTR3;6) in the rice varieties Guihong 1 and Zhonghua 11. Both varieties have a natural one-base mutation in the EBE of another major susceptibility gene (OsSWEET13) which is not induced by the corresponding TALE. Two rice lines GT0105 (from Guihong 1) and ZT0918 (from Zhonghua 11) with target mutations and transgene-free were obtained and showed significantly enhanced resistance to the tested strains of Xoo and Xoc. Furthermore, under simulated field conditions, the morphology and other agronomic traits of GT0105 and ZT0918 were basically the same as those of the wild types.

 

Conclusions: In this study, we first reported that the engineering rice lines obtained by editing the promoters of susceptibility genes are resistant to Xoo and Xoc, and their original agronomic traits are not affected.

 

See: https://pubmed.ncbi.nlm.nih.gov/33891171/

 

Figure 1: CRISPR-mediated editing of the susceptibility genes’ EBEs in rice. a, Schematic presentation of the target sites. The underlined sequences are the target sites. The sequences with black boxes represent the protospacer adjacent motif (PAM). b, Genotypes of the EBEs. Wild type represents the genotypes of Guihong 1 and Zhonghua 11. The sequences underlined in red are TALE binding sites. Deletions are indicated as dashes; the sign −/+ stands for deletion/ insertion; the number represents the number of deletion/insertion bases. The genotypes of OsSWEET11’s EBE in ZT0918 is shown in Supplemental Fig. 2.

Trở lại      In      Số lần xem: 63

[ Tin tức liên quan ]___________________________________________________

 

Designed & Powered by WEBSO CO.,LTD