Dang Minh Tam, Cuu Long Delta Rice Research Institute
PhD Thesis (Plant Breeding), UPLP, The Philippines, and IRRI
e-mail: minhtamdang78@gmail.com
Abstract:
Rice ragged stunt virus (RRSV) and rice grassy stunt virus (RGSV) have been causing serious damages to rice production in Southeast Asian countries. RRSV and RGSV are transmitted by the brown planthopper vector (Delphacidae: Nilaparvata lugens). To identify genetic resources resistance to RGSV and RRSV, five hundred and seventy one traditional rice cultivars and seventy nine wild rice species were evaluated for reactions to RGSV, RRSV and BPH in Vietnam, Thailand and IRRI. Among the traditional cultivars examined, AC1613 showed strong resistance to BPH through antibiosis test in reducing the incidence of RRSV; while nine accessions of wild rice species including Oryza punctata, Oryza officinalis and Oryza minuta resistant to RRSV and RGSV. Mylar cage method for BPH resistance is better than seedling bulk test method and nymph mortality is proposed as an optimized phenotyping method for BPH resistance on AC1613. F1 and segregating F2 and F3 populations from the cross of IR64 and AC1613 were developed for mapping by polymorphic simple sequence repeat (SSR) markers and evaluated for RRSV infection rate and BPH nymph mortality. One major QTL, qRRSV_4 at the map position 100cM, was constructed for RRSV resistance and one major QTL, qNMBPH_4 at the map position 101cM, was identified for resistance to BPH. The two QTLs were located on the same location in short arm of chromosome 4 between RM16281 and RM16284 with the nearest peak marker RM16282. Both BPH and RRSV resistant genes were controlled by single dominant genes. The results explained for a better understanding of how BPH resistance and RRSV resistance expressed on AC1613, thereby promoting the breeding program against RRSV and BPH.
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[ Tin tức liên quan ]___________________________________________________ -
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