Independence Award
- First Rank - Second Rank - Third Rank
Labour Award
- First Rank - Second Rank -Third Rank
National Award
- Study on food stuff for animal(2005)
- Study on rice breeding for export and domestic consumption(2005)
VIFOTEC Award
- Hybrid Maize by Single Cross V2002 (2003)
- Tomato Grafting to Manage Ralstonia Disease(2005)
- Cassava variety KM140(2010)
Curently online : 9 | |
Total visitors : 8284966 | |
Fine mapping and functional validation of the candidate gene BhGA2ox3 for fruit pedicel length in wax gourd (Benincasa hispida)
Monday, 2024/11/25 | 08:13:52
|
||||||||||||||||||||||||||||||||||||||||
Yan Deng, Peng Wang, Wenhui Bai, Zhihao Chen, Zhikui Cheng, Liwen Su, Xianglei Chen, Yeshun Bi, Rongjin Feng & Zhengguo Liu Theoretical and Applied Genetics; November 18 2024; vol. 137; article 272 Key messageThe gene regulating fruit pedicel length in wax gourd was finely mapped to a 211 kb region on chromosome 8. The major gene, Bch08G017310 (BhGA2ox3), was identified through forward genetics. AbstractFruit pedicel length (FPL) is a crucial trait in wax gourd (Benincasa hispida) that affects fruit development and cultivation management. However, the key regulatory genes and mechanisms of FPL in wax gourds remain poorly understood. In this study, we constructed an F2 population using wax gourd plants with long fruit pedicels (GF-7-1-1) and short fruit pedicels (YSB-1-1-2) as parents. Through BSA-seq, we initially localised the FPL candidate gene to an 8.4 Mb region on chromosome 8, which was further narrowed down to a 1.1 Mb region via linkage analysis. A large F2 population of 2163 individuals was used to screen for recombinants, and the locus was ultimately narrowed to within a 211 kb (62,299,856–62,511,174 bp) region. Sequence and expression analyses showed that Bch08G017310 (named BhGA2ox3) is a strong candidate gene for FPL in wax gourds. It encodes gibberellin (GA) 2-beta-dioxygenase, a member of the GA 2-oxidase (GA2ox) family. Cytology showed that GA treatment significantly elongated the fruit pedicels and enlarged the cells in the plants with short fruit pedicels. Ectopic expression of BhGA2ox3 showed that BhGA2ox3 overexpression in Arabidopsis thaliana resulted in significantly shorter fruit pedicels. This study lays a theoretical foundation for the regulatory mechanism of FPL in wax gourds and molecular breeding.
See https://link.springer.com/article/10.1007/s00122-024-04781-z
|
||||||||||||||||||||||||||||||||||||||||
Back Print View: 65 | ||||||||||||||||||||||||||||||||||||||||
[ Other News ]___________________________________________________
|