Independence Award
- First Rank - Second Rank - Third Rank
Labour Award
- First Rank - Second Rank -Third Rank
National Award
- Study on food stuff for animal(2005)
- Study on rice breeding for export and domestic consumption(2005)
VIFOTEC Award
- Hybrid Maize by Single Cross V2002 (2003)
- Tomato Grafting to Manage Ralstonia Disease(2005)
- Cassava variety KM140(2010)
![]() |
|
![]() |
|
Fine mapping of the Chilli veinal mottle virus resistance 4 (cvr4) gene in pepper (Capsicum annuum L.)
Thursday, 2025/01/09 | 07:52:56
|
||||||||||||||||||||||||||||||||||||||||
Joung-Ho Lee, Jung-Min Kim, Jin-Kyung Kwon & Byoung-Cheorl Kang Theoretical and Applied Genetics; January 7 2025; vol.138; article 19 Key messageThe single recessive Chilli veinal mottle virus resistance locus, cvr4, was fine-mapped in pepper through bulked segregant RNA sequencing combined with gene silencing analysis. AbstractChilli veinal mottle virus (ChiVMV) is a widespread pathogen affecting the production of peppers (Capsicum annuum L.) in Asia and Africa. Few loci conferring resistance to ChiVMV have been identified, severely limiting the development of resistant cultivars. To identify ChiVMV resistance genes, we constructed an F2:3 segregating population derived from a cross between the ChiVMV-resistant cultivar ‘CV9’ and the susceptible cultivar ‘Jeju’. The inheritance study of F2:3 populations showed a 1:3 ratio of resistant to susceptible individuals, demonstrating the existence of a single recessive ChiVMV resistance gene in CV9; we named this gene cvr4. To map the cvr4 locus, we employed bulked segregant analysis by RNA sequencing (BSR-seq) of pools from resistant and susceptible F2:3 individuals. We mapped cvr4 to the telomeric region of pepper chromosome 11. To narrow down the cvr4 locus, we developed additional molecular markers in the cvr4 target region, leading to a 2-Mb region of chromosome 11 showing complete co-segregation with the ChiVMV resistance phenotype. Using the polymorphisms identified during BSR-seq, we defined a list of 15 candidate genes for cvr4, which we tested through virus-induced gene silencing analysis for ChiVMV resistance. Of these, the silencing of several genes (DEM.v1.00021323, DEM.v1.00021336, and DEM.v1.00021337) restricted virus spread. Although DEM.v1.00021323 transcript levels were similar between the resistant and susceptible bulks, its alternative spliced isoforms differed in abundance, suggesting that the splicing variants of DEM.v1.00021323 might affect viral infection. These findings may facilitate the breeding of ChiVMV-resistant cultivars in pepper.
See https://link.springer.com/article/10.1007/s00122-024-04805-8
Fig.3: The cvr4 locus maps to pepper chromosome 11. A Δ(SNP-index) plot calculated from the BSR-seq data for pepper chromosome 11. The red line indicates the average value of Δ(SNP-index) for each window (4 Mb size with 50 kb increment). The blue circles represent individual Δ(SNP-index) values at each chromosomal position. The green and orange lines indicate the 95% and 99% confidence intervals for Δ(SNP-index). B Partial genetic map of the cvr4 locus on chromosome 11. Numbers of the diagram represent the genetic distance of markers, with their names shown. C Recombination map of markers closely linked to the cvr4 locus. Plants homozygous for the CV9 or Jeju allele or heterozygous at the indicated markers are indicated by black, white and gray bars, respectively. Numbers in parentheses above the map represent the number of recombinants for each marker. The phenotypes of F2 individuals were inferred by screening F2-derived F3 individuals
|
||||||||||||||||||||||||||||||||||||||||
![]() ![]() ![]() |
||||||||||||||||||||||||||||||||||||||||
[ Other News ]___________________________________________________
|