Welcome To Website IAS

Hot news
Achievement

Independence Award

- First Rank - Second Rank - Third Rank

Labour Award

- First Rank - Second Rank -Third Rank

National Award

 - Study on food stuff for animal(2005)

 - Study on rice breeding for export and domestic consumption(2005)

VIFOTEC Award

- Hybrid Maize by Single Cross V2002 (2003)

- Tomato Grafting to Manage Ralstonia Disease(2005)

- Cassava variety KM140(2010)

Centres
Website links
Vietnamese calendar
Library
Visitors summary
 Curently online :  1
 Total visitors :  5114303

Genomic assay reveals tolerance of DNA damage by both translesion DNA synthesis and homology-dependent repair in mammalian cells
Thursday, 2013/04/18 | 09:47:23

Lior Izhara, Omer Ziva, Isadora S. Cohena, Nicholas E. Geacintovb, and Zvi Livneha,1

 

Author Affiliations

 

1.aDepartment of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel; and
2.bChemistry Department, New York University, New York, NY 10003-5180
1. Edited by Philip C. Hanawalt, Stanford University, Stanford, CA, and approved February 26, 2013 (received for review October 8, 2012)

 

Abstract

 

DNA lesions can block replication forks and lead to the formation of single-stranded gaps. These replication complications are mitigated by DNA damage tolerance mechanisms, which prevent deleterious outcomes such as cell death, genomic instability, and carcinogenesis. The two main tolerance strategies are translesion DNA synthesis (TLS), in which low-fidelity DNA polymerases bypass the blocking lesion, and homology-dependent repair (HDR; postreplication repair), which is based on the homologous sister chromatid. Here we describe a unique high-resolution method for the simultaneous analysis of TLS and HDR across defined DNA lesions in mammalian genomes. The method is based on insertion of plasmids carrying defined site-specific DNA lesions into mammalian chromosomes, using phage integrase-mediated integration. Using this method we show that mammalian cells use HDR to tolerate DNA damage in their genome. Moreover, analysis of the tolerance of the UV light-induced 6–4 photoproduct, the tobacco smoke-induced benzo[a]pyrene-guanine adduct, and an artificial trimethylene insert shows that each of these three lesions is tolerated by both TLS and HDR. We also determined the specificity of nucleotide insertion opposite these lesions during TLS in human genomes. This unique method will be useful in elucidating the mechanism of DNA damage tolerance in mammalian chromosomes and their connection to pathological processes such as carcinogenesis.

 

http://www.pnas.org/content/110/16/E1462.abstract.html?etoc

PNAS April 16, 2013 vol. 110 no. 16 E1462-E1469

Back      Print      View: 1432

[ Other News ]___________________________________________________
  • Transgenic pig carrying green fluorescent proteasomes
  • Ammonia oxidation pathways and nitrifier denitrification are significant sources of N2O and NO under low oxygen availability
  • Genetic analysis and mapping of genes for resistance to multiple strains of Soybean mosaic virus in a single resistant soybean accession PI 96983
  • IRRI Scientists Develop Super Salt-tolerant Rice
  • A dominant major locus in chromosome 9 of rice (Oryza sativa L.) confers tolerance to 48°C high temperature at seedling stage.
  • Source-sink dynamics and proteomic reprogramming under elevated night temperature and their impact on rice yield and grain quality
  • Gene similarity networks provide tools for understanding eukaryote origins and evolution
  • Suppression of Arabidopsis protophloem differentiation and root meristem growth by CLE45 requires the receptor-like kinase BAM3
  • Bacillus subtilis biofilm induction by plant polysaccharides
  • Scientists Re-evaluate Nutrient Uptake of Modern Corn
  • A Dominant Major Locus in Chromosome 9 of Rice (Oryza sativa L.) Confers Tolerance to 48 °C High Temperature at Seedling Stage
  • Scientists Identify Proton Pathway in Photosynthesis
  • Scientists Find Way to Increase Phosphorus Content in Wheat
  • De novo next-generation sequencing, assembling and annotation of Arachis hypogaea L. Spanish botanical type whole plant transcriptome
  • Targeted linkage map densification to improve cell wall related QTL detection and interpretation in maize
  • Fine-mapping and molecular marker development for Pi56(t), a NBS-LRR gene conferring broad-spectrum resistance to Magnaporthe oryzae in rice
  • Genome-wide comparative diversity uncovers multiple targets of selection for improvement in hexaploid wheat landraces and cultivars
  • Smoke-derived karrikin perception by the α/β-hydrolase KAI2 from Arabidopsis
  • USDA Approves Testing of Pink Pineapple
  • Characterization and fine mapping of the rice premature senescence mutant ospse1

 

Designed & Powered by WEBSO CO.,LTD