Welcome To Website IAS

Hot news
Achievement

Independence Award

- First Rank - Second Rank - Third Rank

Labour Award

- First Rank - Second Rank -Third Rank

National Award

 - Study on food stuff for animal(2005)

 - Study on rice breeding for export and domestic consumption(2005)

VIFOTEC Award

- Hybrid Maize by Single Cross V2002 (2003)

- Tomato Grafting to Manage Ralstonia Disease(2005)

- Cassava variety KM140(2010)

Centres
Website links
Vietnamese calendar
Library
Visitors summary
 Curently online :  13
 Total visitors :  8718638

Researchers Fine Tune the Ability of Agrobacterium to Engineer Plants and Fungi
Tuesday, 2024/11/19 | 08:13:21

A research team from the lab of Patrick Shih, an investigator at the Innovative Genomics Institute (IGI) at UC Berkeley, the Deputy Vice President of the Feedstocks Division, and the Director of Plant Biosystems Design at Joint BioEnergy Institute (JBEI), made simple changes to Agrobacterium to improve the efficiency of introducing DNA into a genome.

 

Based on previous literature, the research team hypothesized that engineering plasmids with higher copy numbers could lead to more efficient Agrobacterium-mediated transformation (AMT). The team examined four origins of replication used in AMTs, engineered random mutations, and applied a directed evolution assay to select variants with higher copy numbers.

 

Shih explained that, after identifying mutations, the team confirmed that several of these changes indeed increased the copy number. They then incorporated these optimized plasmids into the binary vector used by Agrobacterium to transfer DNA into the plant genome. The team said that the efficiency of transformation was improved by up to 100% in plants and 400% in fungi.

 

“Hopefully plant scientists will look at this and say, maybe there's more that we should be thinking about when we try to optimize these systems and maybe we can now use this tool set to begin tinkering in other species to see what works best,” said Matthew Szarzanowicz, first author of the study.

 

For more information, read the press release from IGI.

See https://www.isaaa.org/kc/cropbiotechupdate/article/default.asp?ID=21081

 

Back      Print      View: 216

[ Other News ]___________________________________________________
  • Australia`s Gene Regulator OGTR Invites Comments on Field Trial of GM Perennial Ryegrass
  • Long Lost Chromosome Increases Nitrogen Efficiency of Modern Maize
  • Modified Agrobacterium Strain Useful for Switchgrass Transformation
  • Study Reveals Role of Soybean 14-3-3 Gene on White Mold Resistance
  • CIMMYT Study Says Breeding New Crops Must Adapt to Climate Change
  • Researchers Identify Genes to Help Fruit Adapt to Droughts
  • Kenyans Need to Turn to GM Crops to Combat Drought
  • 28-Million-Year-Old Gene Protects Plants Against Caterpillars
  • Agronomists Find Wheat Varieties Resistant to Enzyme Depletion
  • Root Structure Mapped Out to Identify Components of Drought Stress Tolerance in Rice
  • Scientists Report First use of CRISPR to Substitute Genes to Treat Patients with Cancer
  • Large Chinese Seed Companies Likely to Produce Gene-Edited Crops for Farmers – Study
  • Study Finds CRISPR-Cas9 Leads to Unexpected Genomic Changes
  • Plants Yield Better When Grown Among Genetically Similar Plants
  • Codex Alimentarius: FAO Director-General stresses key role of science and data in the Commission`s work
  • World Food Programme and ICRISAT: working to improve nutrition and build resilience in vulnerable communities
  • From Lab to Farm: Scientific research and its contribution to family farming and rural entrepreneurship
  • Chemists Create Artificial Photosynthesis 10 Times More Efficient than Existing Systems
  • Micro/Nanofluidics and Lab-on-Chip Based Emerging Technologies for Biomedical and Translational Research Applications - Part B
  • Scientists Identify Wheat Genetically Resistant to Fungus Causing Snow Mold

 

Designed & Powered by WEBSO CO.,LTD