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miR396b/GRF6 module contributes to salt tolerance in rice
Sunday, 2024/12/29 | 06:11:40

Huanran YuanMingxing ChengRuihua WangZhikai WangFengfeng FanWei WangFengfeng SiFeng GaoShaoqing Li

Plant Biotechnol J.; 2024 Aug; 22(8):2079-2092. doi: 10.1111/pbi.14326. 

Abstract

Salinity, as one of the most challenging environmental factors restraining crop growth and yield, poses a severe threat to global food security. To address the rising food demand, it is urgent to develop crop varieties with enhanced yield and greater salt tolerance by delving into genes associated with salt tolerance and high-yield traits. MiR396b/GRF6 module has previously been demonstrated to increase rice yield by shaping the inflorescence architecture. In this study, we revealed that miR396b/GRF6 module can significantly improve salt tolerance of rice. In comparison with the wild type, the survival rate of MIM396 and OE-GRF6 transgenic lines increased by 48.0% and 74.4%, respectively. Concurrent with the increased salt tolerance, the transgenic plants exhibited reduced H2O2 accumulation and elevated activities of ROS-scavenging enzymes (CAT, SOD and POD). Furthermore, we identified ZNF9, a negative regulator of rice salt tolerance, as directly binding to the promoter of miR396b to modulate the expression of miR396b/GRF6. Combined transcriptome and ChIP-seq analysis showed that MYB3R serves as the downstream target of miR396b/GRF6 in response to salt tolerance, and overexpression of MYB3R significantly enhanced salt tolerance. In conclusion, this study elucidated the potential mechanism underlying the response of the miR396b/GRF6 network to salt stress in rice. These findings offer a valuable genetic resource for the molecular breeding of high-yield rice varieties endowed with stronger salt tolerance.

 

See https://pubmed.ncbi.nlm.nih.gov/38454780/

 

Figure 4. ZNF9 functions as a salt stress induced transcriptional activator. (a) Analysis of the C3HC4‐type RING finger domain in ZNF9 protein sequence. The black box indicates the C3HC4‐type RING finger domain. (b) qRT–PCR analysis of the ZNF9 expression in various organs. R, roots; C, culms; L, leaves; YP0, YP1, YP2, YP3, YP4 and YP5, represent young panicles about 0–0.5 cm, 0.5–1 cm, 1–2 cm, 2–3 cm, 3–4 cm, and 4–5 cm, respectively. (c) ZNF9 expression in 14‐day seedlings treated with 200 mM NaCl. (d) Subcellular localization of ZNF9‐sGFP fusion protein in rice protoplasts. D53–mCherry was used as a nuclear marker. Scale bars, 5 μm. (e) Transcriptional activation analysis of ZNF9 in yeast. pGADT7‐T + pGBKT7‐53 and pGADT7‐T + pGBKT7‐Lam were used as positive and negative control, respectively. (f) Schematic diagrams of the effector and reporter used for transcriptional activation analysis in rice protoplasts. (g) Quantitative analysis of ZNF9 transcriptional activation in rice protoplasts. Different asterisks in g indicate significant differences determined by Student's t‐test (***P < 0.001). Data are mean ± SD (n = 3).

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