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CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging
Tuesday, 2018/11/20 | 07:53:10

Nature; Methods, VOL 15 | NOVEMBER 2018 | 928–931 | www.nature.com/naturemethods

 

ABSTRACT

 

Clustered regularly interspaced short palindromic repeats (CRISPR) guide RNA scaffolds have been adapted to carry multiple binding sites for fluorescent proteins to enhance  brightness for live cell imaging of genomic loci. However, many of these modifications result in guide RNA instability and thus produce lower genome-labeling efficiency than anticipated. Here we introduce CRISPR-Sirius, based on octet arrays of aptamers conferring both enhanced guide RNA stability and brightness, and provide initial biological applications of  this platform.

 

See: https://www.nature.com/articles/s41592-018-0174-0.epdf?no_publisher_access=1&r3_referer=nature

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