Research Team Improves Drought and Salt Tolerance in Cotton and Poplar Using Arabidopsis Gene
Monday, 2016/01/11 | 07:42:29
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Researchers led by Lin-Hui Yu from the University of Science and Technology of China, reported that the Arabidopsis ENHANCED DROUGHT TOLERANCE1/ HOMEODOMAIN GLABROUS11 (AtEDT1/HDG11) also confers drought and salt tolerance in cotton (Gossypium hirsutum) and the woody plant poplar (Populus tomentosa Carr.). Although the gene has been known to confer tolerance to crops such as rice and Arabidopsis, this was the first time it was used for both cotton and poplar.
Both the transgenic cotton and poplar exhibited enhanced tolerance to drought and salt stress. Furthermore, the transgenic cotton showed significant improvement in drought tolerance and better agronomic performance with higher cotton yield in the field both under normal and drought conditions.
Their results demonstrate that AtHDG11 is a promising candidate gene for improving drought and salt of crops and woody plants.
For more information on the study, read the article in Plant Biotechnology Journal.
Figure 1: Overexpression vectors and expression analysis of AtHDG11 in the transgenic cotton plants. (a) Schematic representation of the T-DNA region of AtHDG11-overexpressing vector. LB, left border; RB, right border; 35S polyA, cauliflower mosaic virus (CaMV) 35S polyA; NPT, hygromycin phosphotransferase gene; p35S, CaMV 35S promoter; pTUB2, Arabidopsis Tublin2 promoter; Tnos, 3′-termination signal of nopaline synthase. (b) AtHDG11 transcript levels in the transgenic lines, as revealed by RT-PCR analysis. GhHis3 was used as internal control. |
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