Welcome To Website IAS

Hot news
Achievement

Independence Award

- First Rank - Second Rank - Third Rank

Labour Award

- First Rank - Second Rank -Third Rank

National Award

 - Study on food stuff for animal(2005)

 - Study on rice breeding for export and domestic consumption(2005)

VIFOTEC Award

- Hybrid Maize by Single Cross V2002 (2003)

- Tomato Grafting to Manage Ralstonia Disease(2005)

- Cassava variety KM140(2010)

Centres
Website links
Vietnamese calendar
Library
Visitors summary
 Curently online :  20
 Total visitors :  7455715

CRISPR-Cas9-mediated Engineering of the γ-Aminobutyric Acid Pathway in Tomato
Tuesday, 2017/08/15 | 08:03:28

The CRISPR system has become a widely used technique to perform targeted mutagenesis in a variety of species. However, few studies have modified metabolic pathways in plants using the CRISPR system.

 

China Agricultural University researchers, led by Rui Li introduced the pYLCRISPR-Cas9 system, with has one or more single-site guide RNAs to target the γ-aminobutyric acid (GABA) shunt in tomatoes, which has five key genes.

 

The team first tested the efficiency of the pYLCRISPR-Cas9 system in tomatoes by editing the tomato phytoene desaturase gene (slyPDS). It resulted in an albino phenotype, suggesting the pYLCRISPR-Cas9 system's effectiveness in tomato.

 

For metabolic engineering in tomatoes using multiplex CRISPR-Cas9 system, the team developed six guide RNAs that targeted the five key genes. The resulting CRISPR mutants had significantly higher GABA contents in the leaves and fruits than in the wild types. Furthermore, GABA overaccumulation significantly affected plant vegetative and reproductive growth.

 

These results provide new knowledge on the application of multiplex CRISPR-Cas9 genome editing to plant metabolic engineering and reveal the functional role of GABA in tomato growth.

 

For more on this study, read the article in Plant Biotechnology Journal.

Back      Print      View: 1601

[ Other News ]___________________________________________________
  • Beyond genes: Protein atlas scores nitrogen fixing duet
  • 2016 Borlaug CAST Communication Award Goes to Dr. Kevin Folta
  • FAO and NEPAD team up to boost rural youth employment in Benin, Cameroon, Malawi and Niger
  • Timely seed distributions in Ethiopia boost crop yields, strengthen communities’ resilience
  • Parliaments must work together in the final stretch against hunger
  • Empowering women farmers in the polder communities of Bangladesh
  • Depression: let’s talk
  • As APEC Concludes, CIP’s Food Security and Climate Smart Agriculture on Full Display
  • CIAT directly engages with the European Cocoa Industry
  • Breeding tool plays a key role in program planning
  • FAO: Transform Agriculture to Address Global Challenges
  • Uganda Holds Banana Research Training for African Scientists and Biotechnology Regulators
  • US Congress Ratifies Historic Global Food Security Treaty
  • Fruit Fly`s Genetic Code Revealed
  • Seminar at EU Parliament Tackles GM Crops Concerns
  • JICA and IRRI ignites a “seed revolution” for African and Asian farmers
  • OsABCG26 Vital in Anther Cuticle and Pollen Exine Formation in Rice
  • Akira Tanaka, IRRI’s first physiologist, passes away
  • WHO calls for immediate safe evacuation of the sick and wounded from conflict areas
  • Farmer Field School in Tonga continues to break new ground in the Pacific for training young farmers

 

Designed & Powered by WEBSO CO.,LTD