Welcome To Website IAS

Hot news
Achievement

Independence Award

- First Rank - Second Rank - Third Rank

Labour Award

- First Rank - Second Rank -Third Rank

National Award

 - Study on food stuff for animal(2005)

 - Study on rice breeding for export and domestic consumption(2005)

VIFOTEC Award

- Hybrid Maize by Single Cross V2002 (2003)

- Tomato Grafting to Manage Ralstonia Disease(2005)

- Cassava variety KM140(2010)

Centres
Website links
Vietnamese calendar
Library
Visitors summary
 Curently online :  3
 Total visitors :  4779445

Researchers Engineer a Rapid TALEN Assembly Protocol for Genome Editing
Tuesday, 2020/10/06 | 08:33:57

A speedy transcription activator-like effector nuclease (TALEN) preparation protocol was developed based on widely used Golden Gate TALEN and TAL Effector Kit 2.0. This new protocol can be used to prepare any custom 18-bp binding TALENs in about 12 hours, which is faster than CRISPR. The results of the study are published in ACS Publications.

 

According to the research article, the protocol involves the use of a set linear monomers, a final TALE-FokI backbone plasmid, and a pipeline to assemble the ready-to-use TALEN expression plasmid, which were all designed for the study. The TALEN construction pipeline can simply acquire several positive colonies with high efficiency of over 80 percent. Results of the tests involving five NF-κB genes showed that the new protocol has high efficiency, reproducibility, reliability, and applicability. Furthermore, it was shown that the fabricated TALEN has better editing efficiency than CRISPR.

 

Get more details about the protocol in ACS Publications.

Back      Print      View: 36

[ Other News ]___________________________________________________
  • Egypt Holds Workshop on New Biotech Applications
  • UN Agencies Urge Transformation of Food Systems
  • Taiwan strongly supports management of brown planthopper—a major threat to rice production
  • IRRI Director General enjoins ASEAN states to invest in science for global food security
  • Rabies: Educate, vaccinate and eliminate
  • “As a wife I will help, manage, and love”: The value of qualitative research in understanding land tenure and gender in Ghana
  • CIP Director General Wells Reflects on CIP’s 45th Anniversary
  • Setting the record straight on oil palm and peat in SE Asia
  • Why insect pests love monocultures, and how plant diversity could change that
  • Researchers Modify Yeast to Show How Plants Respond to Auxin
  • GM Maize MIR162 Harvested in Large Scale Field Trial in Vinh Phuc, Vietnam
  • Conference Tackles Legal Obligations and Compensation on Biosafety Regulations in Vietnam
  • Iloilo Stakeholders Informed about New Biosafety Regulations in PH
  • Global wheat and rice harvests poised to set new record
  • GM Maize Harvested in Vietnam Field Trial Sites
  • New label for mountain products puts premium on biological and cultural diversity
  • The Nobel Prize in Physiology or Medicine 2016
  • Shalabh Dixit: The link between rice genes and rice farmers
  • People need affordable food, but prices must provide decent livelihoods for small-scale family farmers
  • GM Seeds Market Growth to Increase through 2020 Due to Rise in Biofuels Use

 

Designed & Powered by WEBSO CO.,LTD