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| A CRISPR/Cas9 deletion into the phosphate transporter SlyPHO1;1 reveals its role in phosphate nutrition of tomato seedlings |
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In vascular (Arabidopsis thaliana) and nonvascular (Physcomitrella patens) plants, PHOSPHATE 1 (PHO1) homologs play important roles in the acquisition and transfer of phosphate. The tomato genome contains six genes (SlPHO1;1-SlPHO1;6) homologous to AtPHO1. The six proteins have typical characteristics of the plant PHO1 family, such as the three SPX sub domains in the N-terminal portion and one EXS domain in the C-terminal portion. |
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Panfeng Zhao, Qiuye You, Mingguang Lei PHISIOLOGIA PLANTARUM; First published: 08 December 2018 https://doi.org/10.1111/ppl.12897 ABSTRACTIn vascular (Arabidopsis thaliana) and nonvascular (Physcomitrella patens) plants, PHOSPHATE 1 (PHO1) homologs play important roles in the acquisition and transfer of phosphate. The tomato genome contains six genes (SlPHO1;1-SlPHO1;6) homologous to AtPHO1. The six proteins have typical characteristics of the plant PHO1 family, such as the three SPX sub domains in the N-terminal portion and one EXS domain in the C-terminal portion. Phylogenetic analysis revealed that the SlPHO1 family is subdivided into three clusters. A pairwise comparison indicated that SlPHO1;1 showed the highest level of sequence identity/similarity (67.39/76.21%) to AtPHO1. SlPHO1;1 deletion mutants induced by CRISPR/Cas9 displayed typical phenotypes of Pi starvation, such as decreased shoot fresh weight (SFW) and increased root fresh weight (RFW), therefore having a greater root-to-shoot (R/S) ratio. Mutants also accumulated more anthocyanin and had more soluble Pi content in the root and less in the shoot. These results indicate that SlPHO1;1 plays an important role in Pi transport in the tomato at seedling stage.
See: https://onlinelibrary.wiley.com/doi/abs/10.1111/ppl.12897 |
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