Hai-long Lu, Ge Dong, Hui Hua, Wen-rui Zhao, Jiu-yu Li, Ren-kou Xu

Ecotoxicology and Environmental Safety; Volume 187,  2020, 109813

Abstract

To explore the relationship between charge characteristics of rice roots and aluminum (Al) tolerance of rice, roots of 47 different rice genotypes were obtained by hydroponic experiment. The zeta potentials of roots were determined by streaming potential method, and the Al tolerance and the functional groups of rice were measured by relative root elongation and infrared spectroscopy (ATR-FTIR), respectively. The exchangeable, complexed and precipitated Al(III) sorbed on the root surface of rice was extracted with 1 mol L⁻¹ KNO₃, 0.05 mol L⁻¹ EDTA-2Na and 0.01 mol L⁻¹ HCl, respectively. There was a significant correlation between the zeta potentials and the relative elongation of rice roots, indicating that the zeta potentials of rice roots could be used to characterize rice tolerance to Al toxicity. Twelve Al-tolerant rice varieties, 25 medium Al-tolerant rice varieties, and 10 Al-sensitive rice varieties were obtained. The Al-tolerant rice varieties sorbed less complexed Al(III) and total Al(III) because there was lower negative charge on their roots compared to less tolerant genotypes. A correlation analysis showed that there were significant negative correlations between the zeta potential, relative root elongation, and the total Al(III) sorption capacity of the roots, which further confirmed the reliability of using the root zeta potential to characterize rice tolerance to Al toxicity. The results of this paper provide a new method for screening Al-tolerant rice varieties.

See: https://www.sciencedirect.com/science/article/abs/pii/S0147651319311443?via%3Dihub

Figure: Al-tolerant rice roots carried less negative charges than Al-sensitive rice roots.