Mariel A Pfeifer, Chang Hyun Khang.

Fungal Genet. Biol. 2021 Jan;146:103472.  doi: 10.1016/j.fgb.2020.103472.

Abstract

The arrangement of the nuclear envelope in the rice blast fungus, Magnaporthe oryzae, was previously undetermined. Here, we identified two conserved components of the nuclear envelope, a core nucleoporin, Nup84, and an inner nuclear membrane protein, Src1. Live-cell super-resolution structured illumination microscopy revealed that Nup84-tdTomato and Src1-EGFP colocalized within the nuclear envelope during interphase and that Nup84-tdTomato remained associated with the dividing nucleus. We also found that appressorium development involved a mitotic nuclear migration event through the germ tube.

See: https://pubmed.ncbi.nlm.nih.gov/32980454/

Figure 1: Arrangement of two nuclear envelope components in M. oryzae. (A) Maximum intensity projection of an SR-SIM image of M. oryzae strain CKF3810. A conidium of CKF3810 shows three nuclei in interphase. Nup84-tdTomato (left panel, red) localizes within the nuclear envelope. Nuclear contents are labelled with two constructs, histone H1 (hH1)-EGFP and EGFP-NLS (middle panel, green). Right panel is a merged image. Scale bar = 2 µm. (B) Single z-slice SR-SIM image of CKF3810 nucleus in the early stage of mitosis in the apical cell of the conidium. Nup84-tdTomato (left panel, red) surrounds hH1-EGFP (middle panel, green). A single z-slice image is displayed because it shows hH1-EGFP signal in optimal detail. EGFP-NLS is dispersed from the nucleus (data not shown). Right panel is a merged image. Scale bar = 1 µm. (C) Single z-slice SR-SIM image of CKF3881 nucleus in the apical cell of the conidium in interphase. Nup84-tdTomato (left panel, red) is distributed along the circumference of Src1-EGFP (middle panel, pseudocolored cyan). Right panel is a merged image. Scale bar = 1 µm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)