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| Overexpression of a methyl-CpG-binding protein gene OsMBD707 leads to larger tiller angles and reduced photoperiod sensitivity in rice |
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Methyl-CpG-binding domain (MBD) proteins play important roles in epigenetic gene regulation, and have diverse molecular, cellular, and biological functions in plants. MBD proteins have been functionally characterized in various plant species, including Arabidopsis, wheat, maize, and tomato. In rice, 17 sequences were bioinformatically predicted as putative MBD proteins. However, very little is known regarding the function of MBD proteins in rice. |
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Mengyu Qu, Zhujian Zhang, Tingmin Liang, Peipei Niu, Mingji Wu, Wenchao Chi, Zi-Qiang Chen, Zai-Jie Chen, Shubiao Zhang & Songbiao Chen BMC Plant Biology volume 21, Article number: 100 (18 February 2021) BackgroundMethyl-CpG-binding domain (MBD) proteins play important roles in epigenetic gene regulation, and have diverse molecular, cellular, and biological functions in plants. MBD proteins have been functionally characterized in various plant species, including Arabidopsis, wheat, maize, and tomato. In rice, 17 sequences were bioinformatically predicted as putative MBD proteins. However, very little is known regarding the function of MBD proteins in rice. ResultsWe explored the expression patterns of the rice OsMBD family genes and identified 13 OsMBDs with active expression in various rice tissues. We further characterized the function of a rice class I MBD protein OsMBD707, and demonstrated that OsMBD707 is constitutively expressed and localized in the nucleus. Transgenic rice overexpressing OsMBD707 displayed larger tiller angles and reduced photoperiod sensitivity—delayed flowering under short day (SD) and early flowering under long day (LD). RNA-seq analysis revealed that overexpression of OsMBD707 led to reduced photoperiod sensitivity in rice and to expression changes in flowering regulator genes in the Ehd1-Hd3a/RFT1 pathway. ConclusionThe results of this study suggested that OsMBD707 plays important roles in rice growth and development, and should lead to further studies on the functions of OsMBD proteins in growth, development, or other molecular, cellular, and biological processes in rice.
See: https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-021-02880-3
Figure 2: Phylogenetic analysis of OsMBD707. Phylogenetic tree was constructed using the neighbor-joining algorithm with 1000 bootstrap replicates. OsMBD707 is indicated by a black triangle. Other OsMBDs are indicated by empty triangles. Only the 13 OsMBDs with active expression detected by qRT-PCR were included in the phylogenetic analysis |
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(124).png "Overexpression of a methyl-CpG-binding protein gene OsMBD707 leads to larger tiller angles and reduced photoperiod sensitivity in rice")
