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| Targeted Modification of Rice Genome with Base Editing |
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Based editing is one of the approaches used for genome editing to generate single-nucleotide variants (SNVs) in genomes. The protocol presented is specifically for targeted adenine to guanine substitution in rice using an adenine base editor. A detailed design of the sgRNA, CRISPR plasmid construction, rapid genetic transformation of rice, and genotyping of editing events are discussed. It can also be used for cytosine base editing. |
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Figure: Graphical abstract from STAR Protocols.
Experts from the Chinese Academy of Agricultural Sciences developed an experimental protocol for targeted modification of the rice genome using base editing, which is published in STAR Protocols.
Based editing is one of the approaches used for genome editing to generate single-nucleotide variants (SNVs) in genomes. The protocol presented is specifically for targeted adenine to guanine substitution in rice using an adenine base editor. A detailed design of the sgRNA, CRISPR plasmid construction, rapid genetic transformation of rice, and genotyping of editing events are discussed. It can also be used for cytosine base editing.
Download the complete document from STAR Protocols. https://www.isaaa.org/kc/cropbiotechupdate/ged/article/default.asp?ID=19898 |
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(91).png "Targeted Modification of Rice Genome with Base Editing")
