ADDRESS: BUI TL, Cuu Long Delta Rice Research Institute, Cantho, Vietnam
e-mail: Bui Liem [liem.rice@gmail.com]
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Constitutively expressed ERF-VII transcription factors redundantly activate the coreanaerobic response in Arabidopsis thaliana
Abstract:
Plant adaptation to hypoxic conditions is mediated by the transcriptional activation of genes involved in the metabolic reprogramming of plant cells to cope with reduced oxygen availability. Recent studies indicated that members of the group VII of the Ethylene Responsive Transcription Factor (ERFs) family act as positive regulators of this molecular response. In the current study, the five ERF-VII transcription factors of Arabidopsisthaliana were compared to infer a hierarchy in their role with respect to the anaerobic response. When the activity of each transcription factor was tested on a set of hypoxiaresponsive promoters, RAP2.2, RAP2.3 and RAP2.12 appeared to be the most powerful activators. RAP2.12 was further dissected in transactivation assays in Arabidopsis protoplasts to identify responsible regions for transcriptional activation. An ultimate Cterminal motif was identified as sufficient to drive gene transcription. Finally, using realtime RT-PCR in single and double mutants for the corresponding genes, we confirmed that RAP2.2 and RAP2.12 exert major control upon the anaerobic response.
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Cross taxa analysis of the molecular response to low oxygen conditions in the plant kingdom
Abstract:
Plants adapt to environmental changes by evolutionary transformation not only in morphology physiology strategies but also genetic backgrounds. Low oxygen conditions occur often during a plant’s life cycle due to limitation of oxygen sources, such as during flooding, or high rate of its consumption. ERF-VII transcription factors are key players in regulation of low oxygen responses in Arabidopsis, where these proteins are substrates of the N-end rule pathway. Here, we present the evolutionary responses to low oxygen stress across plant taxa and the identification of roles of ERF-VII transcription factors, their homologs and enzyme components involves in the N-end rule pathway in different plant species. Our results reveal that ERF-VII transcription factors or homologs have been fixed only recently in fern species and maintained in higher plants whereas orthologues of the N-end rule enzymes are conserved in all plant species analyzed. Only spermatophytes displayed consistent upregulation of the genes identified previously as hypoxia responsive in monocots and dicots, whereas their orthologues in less evolved plant species showed fluctuations of these genes. Double mutation of two members ERF-VII RAP2.2 and RAP2.12 represses upregulation of anaerobic genes and overexpression of RAP2.12 could restore these gene responses under anoxia. Interestingly, overexpression of fern ERF homolog could only rescue Hb1 induction but not ADH and PDC in the same experiment. These findings indicate that the N-end rule is widely conserved in plant taxa and the ERF-VII evolved from recently vascular plant Pterisvitata and higher plants. In addition, evolutionary responses to low oxygen in Spermatophytes are consistent to monocot and dicots.
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[ Tin tức liên quan ]___________________________________________________ -
Pham ho Hai. 2011. PhD Thesis Abstract, IAS, Vietnam Study on cassava peels as by-product to feed cattle ( Thứ năm, 10/11/2011 )
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Le Quang Tuyen. 2011. “Genetic analysis for the resistance to small brown planthopper (Laodelphax striatellus Fallén) in two rice varieties”. PhD Thesis Abstract (e-mail: lequangtuyen71@yahoo.com). Sugarcane Research and Development Centre. ( Thứ năm, 10/11/2011 )
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Evaluation of quality genotypes of Oleiferous brassica under different sources and levels of nutrients and their residual effect on succeeding cowpea [Vigna unguiculata (L.) Walp.] (Nguyen Thi Quynh Thuan) ( Thứ năm, 10/11/2011 )
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Biochemical and crystallographic characterization of starch branching enzymes from the Oryza sativa L. seeds (Vu Thi Nhuan, 2008) ( Thứ năm, 10/11/2011 )
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Development of technology for production of value added b-carotene rich mango powder (Nguyen Van Phong, 2007) ( Thứ năm, 10/11/2011 )
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Sonic hedgehog supplementation of oocyte and embryo culture media enhances development of IVF porcine embryos ( Thứ năm, 10/11/2011 )
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Vuong nam trung. 2011. Identification of apparent digestibility energy and amino acid requirements of yorkshire pig at large scale production ( Thứ năm, 02/02/2012 )
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The role of silicon in surface charge characteristics, Phosphorus sorption capacity and aluminium toxicity Of variable charge soils Nguyễn Quang Chơn (Email: chon.nq@iasvn.org) IAS ( Thứ ba, 24/04/2012 )
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Integration of farmers in the shrimp value chain in mekong river delta, Vietnam. PhD. Thesis: Hồ Thị Minh Hợp. 2012.(Académie universitaire wallonie-europe ; université de liège – gembloux agro-bio-tech).Email: mhop75@yahoo.com ( Thứ ba, 24/04/2012 )
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Ha dinh Tuan. 2012. Etiology of sugarcane ratoon stunt disease in Southeast Vietnam and management. PhD Thesis. Nong Lam University. Vietnam. ( Thứ ba, 28/08/2012 )
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Research and development of the upland rice in the cropping systems of Ea Sup district, Daklak province (PhD. Thesis. Dao Minh So, 2012. IAS, Email: so.dm@iasvn.org) ( Thứ sáu, 14/12/2012 )
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Study on early, drought tolerance hybrid maize and cultivation practices in Southern Vietnam (PhD Thesis (2013), Trương Vĩnh Hải, Email: hai.tv@iasvn.org ) ( Thứ ba, 24/06/2014 )
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DINH THI LAM. 2021. Studies on wild genetic resources to improve phosphorus deficiency tolerance in rice (Oryza sativa L.). ( Thứ hai, 08/08/2022 )
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Studying the growth, development capacities and technical measures to increase yields, qualities of some exotic orchid cultivars (Cattleya, Dendrobium, Oncidium) for Northern Vietnam (PhD. Thesis: Hoang Xuan Lam) ( Thứ sáu, 27/06/2014 )
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Research on identifying new varieties and cultivated techniques protocols to improve productivity and economic efficiency in tomato cultivation in Red River Delta (PhD. Thesis. Dang Van Nien) ( Thứ sáu, 27/06/2014 )
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Processed Organic Fertilizer Influences On Soil Fertility Properties And Short- Duration Crop Genotypes’ Productivity Under South-Eastern Grey Soil Conditions. (PhD Thesis. Hoang Van Tam. 2014. The Institute of Agricultural Research for Southern Vietnam) ( Thứ hai, 21/07/2014 )
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Study on P releasing and adsorption in lowland areas of Southern Vietnam and silicate application to raise available P for rice (Oryza sativa L.). (PhD Thesis. TRAN THI TUONG LINH, Submitted in August 14, 2014) ( Thứ năm, 14/08/2014 )
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Research and application of molecule markers in breeding salt-tolerant Bacthom 7 rice variety. ( PhD Thesis. Dong Thi Kim Cuc) ( Thứ năm, 06/11/2014 )
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Study on biological characteristics and technology of spawn production, cultivation of the King oyster mushroom ((Pleurotus eryngii) and Yunzhi mushroom (Trametes versicolor) in Vietnam.( PhD. Thesis. Nguyen Thi Bich Thuy) ( Thứ năm, 06/11/2014 )
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Studying on potassium balance for sugarcane in Lam Son sugarcane belt, Thanh Hoa province.( PhD. Thesis. Pham Thi Thanh Huong) ( Thứ năm, 06/11/2014 )
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