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Gene Editing Enhances Resistance of Potato to Zebra Chip Disease
Wednesday, 2024/06/05 | 08:25:42

A study published in the Plant Biotechnology Journal shows that the genome editing of NPR3 confers potato resistance to Candidatus Liberibacter solanacearum (CLso), a bacterium associated with zebra chip disease. The significant economic losses caused by the disease necessitate alternative approaches to develop resistance to the disease.

 

The researchers generated multiple StNPR3-edited lines using Agrobacterium tumefaciens-mediated transformation. The study found no abnormal growth in edited lines compared to control plants. When evaluated with CLso, the edited lines exhibited reduced disease symptoms, reduced discoloration, a significant reduction in CLso titer, and higher expression of defense-related marker genes.

 

To better understand how the StNPR3-edited potatoes resist disease, the researchers analyzed the changes in gene activity and metabolites. The analysis revealed that many of these genes were involved in processes related to biotic stress and defense responses. Additionally, a mapping of the metabolites showed that several defense-related proteins and transcription factors were activated. This comprehensive analysis helps explain the enhanced disease resistance observed in the StNPR3-edited potatoes.

 

For more information, read the article from the Plant Biotechnology Journal.

See https://www.isaaa.org/kc/cropbiotechupdate/ged/article/default.asp?ID=20832

 

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