Welcome To Website IAS

Hot news
Achievement

Independence Award

- First Rank - Second Rank - Third Rank

Labour Award

- First Rank - Second Rank -Third Rank

National Award

 - Study on food stuff for animal(2005)

 - Study on rice breeding for export and domestic consumption(2005)

VIFOTEC Award

- Hybrid Maize by Single Cross V2002 (2003)

- Tomato Grafting to Manage Ralstonia Disease(2005)

- Cassava variety KM140(2010)

Centres
Website links
Vietnamese calendar
Library
Visitors summary
 Curently online :  7
 Total visitors :  7655189

Genome Editing Plant Cells with Atmospheric Pressure Plasma
Saturday, 2023/03/18 | 07:02:42

A team of researchers from the RIKEN Center for Sustainable Resource Science and partners attempted genome editing using the atmospheric-pressure plasma as protein introduction technique. Their findings are published in an open-access article in PlosOne journal.

 

Transgenic reporter plants were used as an experimental system to assess genome editing. The transgenic plants carried the reporter genes L-(I-SceI)-UC and sGFP-waxy-HPT. The success of genome editing was measured through the chemiluminescent signal from the refunctionalization of the luciferase gene that proceeded after genome editing. In the same way, the sGFP-waxy-HPT system led to hygromycin resistance caused by hygromycin phosphotransferase during genome editing.

 

CRISPR-Cas9 ribonucleoproteins, which target the reporter genes, were introduced into rice calli or tobacco leaves after treatment. Then the rice calli were placed on a suitable medium, leading to a unique luminescence signal. Meanwhile, the tobacco cells carrying the sGFP-waxy-HPT gene showed hygromycin resistance. After recurring cultivation of the treated tobacco leaf pieces on the regeneration medium, the calli grew leaf pieces. Hygromycin-resistant green callus was eventually harvested, and a genome-edited sequence in the tobacco reporter gene was confirmed.

 

Find more details in the study from PlosOne.

https://www.isaaa.org/kc/cropbiotechupdate/ged/article/default.asp?ID=20087

Back      Print      View: 230

[ Other News ]___________________________________________________
  • Brazil offers an extra US $ 17 million to FAO projects as new government takes helm
  • 2014 in review – Another busy year
  • Growing concern for South Sudan`s herders as conflict displaces millions of cattle
  • Biotech and Traditional Farming are Compatible Approaches to Sustainable Agri, Study
  • Report: Weed Control Changes and Herbicide Tolerant Crops in the USA 1996-2012
  • New Study Provides Better Understanding of the Genetic Basis for Drought Tolerant Soybeans
  • Wheat Gene Increases Blight Resistance of American Chestnut Trees
  • China Approves Imports of Biotech Crops
  • IndoBIC Holds Media Visit to Seed Industries in East Java
  • FAO food price index drops in December
  • Origin Receives Biosafety Certificate Renewal for its GM Phytase Corn in China
  • Biotech Rice Expressing CP4-EPSPS Shows Glyphosate Tolerance
  • UK Govt Adviser Calls for Use of Agri Technologies that ``Produce More with Less``
  • Genetic diversity a hidden tool in coping with climate change
  • Cutting down on Amazon deforestation: Watch, think, and act
  • USDA Deregulates Dicamba-Tolerant Cotton and Soybean
  • NAS Holds Workshop on Communicating about GMOs
  • Cell Wall Traits for a FHB Resistant Durum Wheat
  • Ag Biotech Vietnam Conducts Biotech Quiz Contest at Northwestern University
  • Viet Nam Launches National Zero Hunger Challenge

 

Designed & Powered by WEBSO CO.,LTD