Macrophages are immune cells equipped with multiple double-stranded RNA (dsRNA) sensors designed to detect viral infection and amplify innate antiviral immunity. However, many coronaviruses can infect and propagate in macrophages without activating dsRNA sensors. Here we present a function of murine coronavirus nonstructural protein 15 in preventing detection of viral dsRNA by host sensors.

Methane released from the seafloor and transported to the atmosphere has the potential to amplify global warming. At an arctic site characterized by high methane flux from the seafloor, we measured methane and carbon dioxide (CO₂) exchange across the sea−air interface. We found that CO₂ uptake in an area of elevated methane efflux was enhanced relative to surrounding waters, such that the negative radiative forcing effect (cooling)

Pectate lyase genes have been documented as excellent candidates for improvement of fruit firmness. However, implementation of pectate lyase in regulating fruit postharvest deterioration has not been fully explored. In this report, 22 individual pectate lyase genes in tomato were identified, and one pectate lyase gene SlPL (Solyc03g111690) showed dominant expression during fruit maturation.

The growing human population generates increasing demand for food and energy. Microalgae are a promising source of sustainable bioproducts whose production may not exacerbate worsening environmental problems. The green alga Chromochloris zofingiensis has potential as a biofuel feedstock and source of high-value nutraceutical molecules, including the carotenoid astaxanthin.We present a high-quality, chromosome-level assembly of the genome by using a hybrid sequencing approach with independent validation by optical mapping.

Cold and drought stresses seriously affect cassava (Manihot esculenta) plant growth and yield. Recently, long noncoding RNAs (lncRNAs) have emerged as key regulators of diverse cellular processes in mammals and plants. To date, no systematic screening of lncRNAs under abiotic stress and their regulatory roles in cassava has been reported. In this study, we present the first reference catalog of 682 high-confidence lncRNAs based on analysis of strand-specific RNA-seq data from cassava shoot apices and young leaves under cold,

The coffee berry borer (CBB) Hypothenemus hampei is the most limiting pest of coffee production worldwide. The CBB genome has been recently sequenced; however, information regarding the presence and characteristics of transposable elements (TEs) was not provided. Using systematic searching strategies based on both de novo and homology-based approaches, we present a library of TEs from the draft genome of CBB sequenced by the Colombian Coffee Growers Federation.

Bambara groundnut (Vigna subterranea (L.) Verdc.) is an underutilised legume crop, which has long been recognised as a protein-rich and drought-tolerant crop, used extensively in Sub-Saharan Africa. The aim of the study was to identify quantitative trait loci (QTL) involved in agronomic and drought-related traits using an expression marker-based genetic map based on major crop resources developed in soybean.

Parasitic plants are pests of many plants, including major crop species. An important step toward creating resistance to parasitic plants is gaining a better understanding of how these pathogens control the physiology and development of their hosts. We combined genetic, cell-biological

Plant microRNAs (miRNAs) control intricate gene regulatory networks and have been implicated in important developmental switches and stress responses. Plant miRNAs have recently emerged as promising targets for crop improvement because they can control complex agronomic traits; however, functional studies using reverse genetics have been hampered by practical difficulties.

The CRISPR-Cas systems provide invader defense in a wide variety of prokaryotes, as well as technologies for many powerful applications. The Type III-A or Csm CRISPR-Cas system is one of the most widely distributed across prokaryotic phyla, and cleaves targeted DNA and RNA molecules. In this work, we have constructed modules of Csm systems from 3 bacterial species and heterologously expressed the functional modules in E. coli.