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 - Study on food stuff for animal(2005)

 - Study on rice breeding for export and domestic consumption(2005)

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- Hybrid Maize by Single Cross V2002 (2003)

- Tomato Grafting to Manage Ralstonia Disease(2005)

- Cassava variety KM140(2010)

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Saturday, 2024/02/17 | 08:17:51

Chilling tolerance in crops can increase resilience through longer growing seasons, drought escape, and nitrogen use efficiency. In sorghum (Sorghum bicolor [L.] Moench), breeding for chilling tolerance has been stymied by coinheritance of the largest-effect chilling tolerance locus, qSbCT04.62, with the major gene underlying undesirable grain proanthocyanidins, WD40 transcriptional regulator Tannin1. To test if this coinheritance is due to antagonistic pleiotropy of Tannin1, we developed and studied near-isogenic lines (NILs) carrying chilling tolerant haplotypes at qCT04.62.

Friday, 2024/02/16 | 07:22:08

Aphanomyces root rot, caused by Aphanomyces euteiches, is the most important disease of pea (Pisum sativum L.) worldwide. The development of pea-resistant varieties is a major challenge to control the disease. Previous linkage studies identified seven main resistance quantitative trait loci (QTL), including the QTL Ae-Ps4.5 associated with partial resistance in US nurseries infested by the pea pathotype III of A. euteiches. This study aimed to confirm the major effect of Ae-Ps4.5 on A. euteiches pathotype III, refine its interval, and identify candidate genes underlying the QTL.

Thursday, 2024/02/15 | 06:20:06

Induced mutations accelerate crop improvement by providing novel disease resistance and yield alleles. However, the alleles with no perceptible phenotype but have an altered function remain hidden in mutagenized plants. The whole-genome sequencing (WGS) of mutagenized individuals uncovers the complete spectrum of mutations in the genome. Genome-wide induced mutation resources can improve the targeted breeding of tomatoes and facilitate functional genomics. In this study, we sequenced 132 doubly ethyl methanesulfonate (EMS)-mutagenized lines of tomato and detected approximately 41 million novel mutations and 5.5 million short InDels not present in the parental cultivar.

Wednesday, 2024/02/14 | 05:06:32

Epigenetics-mediated breeding (Epibreeding) involves engineering crop traits and stress responses through targeted manipulation of key epigenetic features to enhance agricultural productivity. While conventional breeding methods raise concerns about reduced genetic diversity, epibreeding propels crop improvement through epigenetic variations that regulate gene expression, ultimately impacting crop yield. Epigenetic regulation in crops encompasses various modes, including histone modification, DNA modification, RNA modification, non-coding RNA, and chromatin remodeling.

Tuesday, 2024/02/13 | 07:44:35

The amount of free asparagine in grain of a wheat genotype determines its potential to form harmful acrylamide in derivative food products. Here, we explored the variation in the free asparagine, aspartate, glutamine and glutamate contents of 485 accessions reflecting wheat worldwide diversity to define the genetic architecture governing the accumulation of these amino acids in grain. Accessions were grown under high and low nitrogen availability and in water-deficient and well-watered conditions, and plant and grain phenotypes were measured.

Monday, 2024/02/12 | 06:52:17

The formation of root system architecture (RSA) plays a crucial role in plant growth. OsDRO1 is known to have a function in controlling RSA in rice, however, the role of potato StDRO2, a homolog of rice OsDRO1, in root growth remains unclear. In this study, we obtained potato dro2 mutant lines by Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR-Associated 9 (CRISPR/Cas9)-mediated genome editing system. The mutant lines were generated from a splicing defect of the StDRO2 intron 1, which causes a nonsense mutation in StDRO2. Furthermore, the secondary structure of StDRO2 mRNA analyzed with RNAfold WebServer was altered in the dro2 mutant.

Sunday, 2024/02/11 | 06:57:45

B-cell lymphoma 2 (Bcl-2)-associated athanogene (BAG) family genes play prominent roles in regulating plant growth, development, and stress response. Although the molecular mechanism underlying BAG's response to abiotic stress has been studied in Arabidopsis, the function of OsBAG underlying saline-alkaline stress tolerance in rice remains unclear. In this study, OsBAG6, a chaperone regulator localized to mitochondria, was identified as a novel negative regulator of saline-alkaline stress tolerance in rice. The expression level of OsBAG6 was induced by high concentration of salt, high pH, heat and abscisic acid treatments.

Saturday, 2024/02/10 | 06:31:41

Leaf color is a highly important agronomic trait, and mutants with altered leaf coloration can serve as excellent models for studies on chloroplast development and chlorophyll biosynthesis, enabling the cloning of genes involved in these processes in rice (Oryza sativa L.). In this study, we isolated a stable genetic rice mutant, oryza sativa albino leaf 50 (osal50), from a breeding population of the japonica cultivar GP50. This mutant exhibited a distinctive albino phenotype, with white-striped leaves in seedlings and white panicles at the heading stage. Compared with wild-type GP50, the osal50 mutant showed lower chlorophyll and carotenoid accumulation, together with abnormal chloroplast ultrastructure.

Friday, 2024/02/09 | 07:15:46

Phenotypic plasticity is an important topic in biology and evolution. However, how to generate broadly applicable insights from individual studies remains a challenge. Here, with flowering time observed from a large geographical region for sorghum and rice genetic populations, we examine the consistency of parameter estimation for reaction norms of genotypes across different subsets of environments and searched for potential strategies to inform the study design. Both sample size and environmental mean range of the subset affected the consistency. The subset with either a large range of environmental mean or a large sample size resulted in genetic parameters consistent with the overall pattern.

Thursday, 2024/02/08 | 07:26:36

The development of cereal crops with high nitrogen use efficiency (NUE) is a priority for worldwide agriculture. In addition to conventional plant breeding and genetic engineering, the use of the plant microbiome offers another approach to improving crop NUE. To gain insight into the bacterial communities associated with sorghum lines that differ in NUE, a field experiment was designed comparing 24 diverse Sorghum bicolor lines under sufficient and deficient nitrogen (N). Amplicon sequencing and untargeted gas chromatography-mass spectrometry were used to characterize the bacterial communities and the root metabolome associated with sorghum genotypes varying in sensitivity to low N.

 

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