The two-line hybrid rice system, a cutting-edge hybrid rice breeding technology, has greatly boosted global food security. In thermo-sensitive genic male sterile (TGMS) lines, the critical sterility-inducing temperature (CSIT; the temperature at which TGMS lines change from male fertile to complete male sterile) acts as a key threshold. We recently uncovered that thermo-sensitive genic male sterility 5 (tms5), a sterile locus presenting in over 95% of TGMS lines

Tomato leaf curl New Delhi virus (ToLCNDV), a begomovirus, that causes severe leaf curling, stunting, and reduced yield in tomato plants is consistently threatening its production worldwide. CRISPR/Cas9-mediated genome editing has shown immense potential in developing disease-resistant crops. This study successfully focuses on designing a precise and efficient strategy for in planta defence against ToLCNDV

Submergence stress is a major obstacle limiting the application of direct seeding in rice cultivation. Rapid bud and root growth helps plants establish a stronger growth base and improve their submergence tolerance. Therefore, mining genes for bud length (BL) and root length (RL) helps in the development of varieties that are adaptable to submergence and improve seedling emergence and yield of direct-seeded rice

Cassava is an important root crop that is produced by smallholder farmers across Sub-Saharan Africa. Cassava mosaic disease (CMD), which is caused by a group of cassava mosaic begomoviruses (CMBs), is one of the most devastating diseases of cassava. A previous study showed that SEGS-1 (sequences enhancing geminivirus symptoms), which occur both in the cassava genome and as episomes during CMD, can increase CMD disease severity and overcome host resistance. In this report, we examined the effects of exogenously applied SEGS-1 on the incidence of CMB infection, symptom severity, and viral DNA copy number in five cassava cultivars that ranged from highly susceptible to highly resistant to CMD.

Centuries of clonal propagation in cassava (Manihot esculenta) have reduced sexual recombination, leading to the accumulation of deleterious mutations. This has resulted in both inbreeding depression affecting yield and a significant decrease in reproductive performance, creating hurdles for contemporary breeding programs. Cassava is a member of the Euphorbiaceae family, including notable species such as rubber tree (Hevea brasiliensis) and poinsettia (Euphorbia pulcherrima). Expanding upon preliminary draft genomes, we annotated 7 long-read genome assemblies and aligned a total of 52 genomes, to analyze selection across the genome and the phylogeny.

Springtails (Collembola) are an integral and beneficial component of soil communities. As part of a comprehensive study of the effects of the Bacillus thuringiensis-maize hybrid DKC4442YG (Bt event MON810) expressing Cry1Ab toxin on nontarget invertebrates, we studied abundance and diversity of surface-active springtails in the temperate region of central Europe (western Slovakia) over 3 years in 10 plots with Bt and 10 plots with the near-isogenic non-Bt event DK440.

Controlled-release nitrogen fertilizers are gaining popularity in rice (Oryza stavia L.) cultivation for their ability to increase yields while reducing environmental impact. Grain filling is essential for both the yield and quality of rice. However, the impact of controlled-release nitrogen fertilizer on grain-filling characteristics, as well as the relationship between these characteristics and rice quality, remains unclear.

In recent years, the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technology has been extensively applied in major crops, including rice, wheat, and potato. This technology can be utilized to regulate or disrupt gene expression to develop high-quality, disease-resistant, or stress-tolerant crops (Gao, 2021). However, the current low regeneration efficiency of B. oleracea has led to a limited Agrobacterium-mediated transformation efficiency of less than 1.0%, consequently impacting CRISPR/Cas9 based gene editing efficiency

The stinkbug Riptortus pedestris, notorious for inducing soybean staygreen-like syndrome, employs a range of salivary proteins to manipulate the host plant for its benefit. Here, we show that RpSP1, a salivary protein specific to Pentatomomorpha, triggers plant defense responses in multiple plant species. RpSP1 interacts with and stabilizes a HSP40 family protein GmSPIP1 and is dependent on GmSPIP1 to induce cell death.

The effects of salt stress on plant development and progression are substantial. Ethylene response factor transcription factors (TFs) play a crucial role in responses to salt stress. Their functions in the salt response, particularly in pepper, are still mostly unknown. This study revealed the function of CaERF2 in salt tolerance of pepper plants. CaERF2 expression was dramatically increased in pepper plants following salt stress treatment