Welcome To Website IAS

Hot news
Achievement

Independence Award

- First Rank - Second Rank - Third Rank

Labour Award

- First Rank - Second Rank -Third Rank

National Award

 - Study on food stuff for animal(2005)

 - Study on rice breeding for export and domestic consumption(2005)

VIFOTEC Award

- Hybrid Maize by Single Cross V2002 (2003)

- Tomato Grafting to Manage Ralstonia Disease(2005)

- Cassava variety KM140(2010)

Centres
Website links
Vietnamese calendar
Library
Visitors summary
 Curently online :  24
 Total visitors :  7730346

Premeiotic 24-nt phasiRNAs are present in the Zea genus and unique in biogenesis mechanism and molecular function
Monday, 2024/05/27 | 08:12:12

Junpeng Zhan, Sébastien Bélanger, Scott Lewis, Chong Teng, Madison McGregor, Aleksandra Beric, Michael A. Schon, Michael D. Nodine, and Blake C. Meyers

 

PNAS May 13, 2024; 121 (21) e2402285121

Significance

We previously reported two classes of reproductive phasiRNAs (phased, small interfering RNAs) in maize, the premeiotic 21-nt (nucleotides) phasiRNAs and the meiotic 24-nt phasiRNAs. Here, we report a third class of reproductive phasiRNAs—premeiotic 24-nt phasiRNAs—that are present in the Zea genus, including all five maize inbred lines and three teosinte species/subspecies that we examined, plus rice. We show that in the Zea genus, the premeiotic 24-nt phasiRNAs are distinct from the meiotic 24-nt phasiRNAs in triggering mechanism, effector protein, and molecular function.

Abstract

Reproductive phasiRNAs (phased, small interfering RNAs) are broadly present in angiosperms and play crucial roles in sustaining male fertility. While the premeiotic 21-nt (nucleotides) phasiRNAs and meiotic 24-nt phasiRNA pathways have been extensively studied in maize (Zea mays) and rice (Oryza sativa), a third putative category of reproductive phasiRNAs–named premeiotic 24-nt phasiRNAs–have recently been reported in barley (Hordeum vulgare) and wheat (Triticum aestivum). To determine whether premeiotic 24-nt phasiRNAs are also present in maize and related species and begin to characterize their biogenesis and function, we performed a comparative transcriptome and degradome analysis of premeiotic and meiotic anthers from five maize inbred lines and three teosinte species/subspecies. Our data indicate that a substantial subset of the 24-nt phasiRNA loci in maize and teosinte are already highly expressed at the premeiotic phase. The premeiotic 24-nt phasiRNAs are similar to meiotic 24-nt phasiRNAs in genomic origin and dependence on DCL5 (Dicer-like 5) for biogenesis, however, premeiotic 24-nt phasiRNAs are unique in that they are likely i) not triggered by microRNAs, ii) not loaded by AGO18 proteins, and iii) not capable of mediating PHAS precursor cleavage. In addition, we also observed a group of premeiotic 24-nt phasiRNAs in rice using previously published data. Together, our results indicate that the premeiotic 24-nt phasiRNAs constitute a unique class of reproductive phasiRNAs and are present more broadly in the grass family (Poaceae) than previously known.

 

See https://www.pnas.org/doi/10.1073/pnas.2402285121

 

Figure 4: Total sRNA-seq and TraPR sRNA-seq analyses of the ago18 triple mutant anthers. (A) Mean-difference (MA) plots of PHASloci based on sRNA-seq. (B) Total abundance (mean ± SE) of meiotic 24-nt phasiRNAs in ago18 triple homozygous mutant plants (ago18-HM) and their triple heterozygous siblings (ago18-HT). Results of Student’s t test are shown in SI Appendix, Fig. S13. (C) MA plots from differential accumulation analyses of phasiRNA abundance per loci based on TraPR sRNA-seq. In A and C, average CPM values were calculated for only triple heterozygous samples. Significantly differentially expressed loci (FC > 1.5, FDR < 0.05) are represented by red circles/triangles/plus signs, and the other loci in blue. Dash lines indicate FC = ±1.5 (i.e., log2FC = ±0.585).

 

Back      Print      View: 75

[ Other News ]___________________________________________________
  • Auxin depletion from leaf primordia contributes to organ patterning
  • Phytochrome controls alternative splicing to mediate light responses in Arabidopsis
  • Maternal temperature history activates Flowering Locus T in fruits to control progeny dormancy according to time of year
  • Identification and evaluation of quantitative trait loci underlying resistance to multiple HG types of soybean cyst nematode in soybean PI 437655
  • Factor analytic mixed models for the provision of grower information from national crop variety testing programs
  • Exploring the areas of applicability of whole genome prediction methods for Asian rice (Oryza sativa L.)
  • Investigation of terpene diversification across multiple sequenced plant genomes
  • Arabidopsis ribosomal proteins control vacuole trafficking and developmental programs through the regulation of lipid metabolism
  • Effect of the ahas Transgene on Biological Nitrogen Fixation and Yield of Soybean
  • Arabidopsis ROCK1 transports UDP-GlcNAc/UDP-GalNAc and regulates ER protein quality control and cytokinin activity
  • A peripheral endocannabinoid mechanism contributes to glucocorticoid-mediated metabolic syndrome
  • High-Resolution Linkage Map and Chromosome-Scale Genome Assembly for Cassava (Manihot esculenta Crantz) from 10 Populations
  • Analysis of the Transcriptome of Banana Fruit during Ripening
  • Nitric oxide negatively regulates abscisic acid signaling in guard cells by S-nitrosylation of OST1
  • Salt Tolerant Gene in Soybean Identified
  • Climate change decouples oceanic primary and export productivity and organic carbon burial
  • Evolution of the H9N2 influenza genotype that facilitated the genesis of the novel H7N9 virus
  • 5-Hydroxymethylcytosine Is Not Present in Appreciable Quantities in Arabidopsis DNA
  • Fine mapping of the qLOP2 and qPSR2 1 loci associated with chilling stress tolerance of wild rice seedlings
  • Natural diversity in daily rhythms of gene expression contributes to phenotypic variation

 

Designed & Powered by WEBSO CO.,LTD