Lu Yang, Wei Huan, Fangjie Xiong, Zhiqiang Xian, Deding Su, Maozhi Ren, Zhengguo Li
Plant Biotechnology Journal, 16 May 2017; DOI: 10.1111/pbi.12737
Summary
Pectate lyase genes have been documented as excellent candidates for improvement of fruit firmness. However, implementation of pectate lyase in regulating fruit postharvest deterioration has not been fully explored. In this report, 22 individual pectate lyase genes in tomato were identified, and one pectate lyase gene SlPL (Solyc03g111690) showed dominant expression during fruit maturation. RNA interference of SlPL resulted in enhanced fruit firmness and changes in pericarp cells. More importantly, the SlPL-RNAi fruit demonstrated greater antirotting and pathogen-resisting ability. Compared to wild-type, SlPL-RNAi fruit had higher levels of cellulose and hemicellulose, whereas the level of water-soluble pectin was lower. Consistent with this, the activities of peroxidase, superoxide dismutase and catalase were higher in SlPL-RNAi fruit, and the malondialdehyde concentration was lower. RNA-Seq results showed large amounts of differentially expressed genes involved in hormone signalling, cell wall modification, oxidative stress and pathogen resistance. Collectively, these data demonstrate that pectate lyase plays an important role in both fruit softening and pathogen resistance. This may advance knowledge of postharvest fruit preservation in tomato and other fleshy fruit.
See http://onlinelibrary.wiley.com/doi/10.1111/pbi.12737/full
Figure 1: Phylogenetic analysis, multiple sequence alignment and expression patterns of pectate lyases (PLs). (a) Phylogenetic analysis of tomato PL gene family members, together with all 26 ArabidopsisPLs and two softening-related PLs from strawberry (FaPEL) and banana (MaPEL), all of which were carried out by the neighbour-joining method on MEGA6. (b) Three typical motifs (motif 1 WIDH, motif 2 DGLIDAIMASTAITISNNYF and motif 3 LIQRMPRCRHGYFHVVNNDY) of the PL amino acid sequences from tomato, Arabidopsis, strawberry and banana, comparatively analysed by Clustal X (Thompson et al., 2002). (c) Expression patterns of the tomato PL genes obtained from the TomExpress platform.
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