Wu Y, Xiao N, Chen Y, Yu L, Pan C, Li Y, Zhang X, Huang N, Ji H, Dai Z, Chen X, Li A.

Rice (N Y). 2019 Mar 1;12(1):11. doi: 10.1186/s12284-019-0264-3.

Abstract

BACKGROUND:

Broad-spectrum resistance gene pyramiding helps the development of varieties with broad-spectrum and durable resistance to M. oryzae. However, detailed information about how these different sources of broad-spectrum resistance genes act together or what are the best combinations to achieve broad-spectrum and durable resistance is limited.

RESULTS:

Here a set of fifteen different polygene pyramiding lines (PPLs) were constructed using marker-assisted selection (MAS). Using artificial inoculation assays at seedling and heading stage, combined with natural induction identification under multiple field environments, we evaluated systematically the resistance effects of different alleles of Piz locus (Pigm, Pi40, Pi9, Pi2 and Piz) combined with Pi1, Pi33 and Pi54, respectively, and the interaction effects between different R genes. The results showed that the seedling blast and panicle blastresistance levels of PPLs were significantly higher than that of monogenic lines. The main reason was that most of the gene combinations produced transgressive heterosis, and the transgressive heterosis for panicle blast resistance produced by most of PPLs was higher than that of seedling blast resistance. Different gene pyramiding with broad-spectrum R gene produced different interaction effects, among them, the overlapping effect (OE) between R genes could significantly improve the seedling blast resistance level of PPLs, while the panicle blastresistance of PPLs were remarkably correlated with OE and complementary effect (CE). In addition, we found that gene combinations, Pigm/Pi1, Pigm/Pi54 and Pigm/Pi33 displayed broad-spectrum resistance in artificial inoculation at seedling and heading stage, and displayed stable broad-spectrum resistance under different disease nursery. Besides, agronomic traits evaluation also showed PPLs with these three gene combinations were at par to the recurrent parent. Therefore, it would provide elite gene combination model and germplasms for rice blast resistance breeding program.

CONCLUSIONS:

The development of PPLs and interaction effect analysis in this study provides valuable theoretical foundation and innovative resources for breeding broad-spectrum and durable resistant varieties.

See https://www.ncbi.nlm.nih.gov/pubmed/30825053

Figure 2: Resistance performances of PPLs for seedling and panicle blast resistance. a Correlation analysis of seedling blast resistance with panicle blast resistance of NILs, b Correlation analysis of seedling blast resistance with panicle blast resistance of PPLs, c Comprehensive comparative analysis on seedling blast RF of PPLs, NILs and the recurrent parent, d Comprehensive comparative analysis on panicle blast RF of PPLs, NILs and the recurrent parent, e transgressive heterosis for seedling blast and panicle blast resistance produced by PPLs after different broad-spectrum R gene pyramided.