Bin Li, Shengxü Fan, Fukuan Yu, Ying Chen, Shengrui Zhang, Fenxia Han, Shurong Yan, Lianzheng Wang, Junming Sun

Theoretical and Applied Genetics; July 2017, Volume 130, Issue 7, pp 1467–1479

Abstract

Key message

We constructed a high-density linkage map comprising 3541 markers developed by specific-locus amplified fragment sequencing, and identified 26 stable QTL including nine novel loci, for fatty acid composition in soybean.

Abstract

Soybean oil quality and stability are mainly determined by the fatty acid composition of the seed. In the present study, we constructed a high-density genetic linkage map using 200 recombinant inbred lines derived from a cross between cultivated soybean varieties Luheidou2 and Nanhuizao, and SNP markers developed by specific-locus amplified fragment sequencing (SLAF-seq). This map comprises 3541 markers on 20 linkage groups and spans a genetic distance of 2534.42 cM, with an average distance of 0.72 cM between adjacent markers. Inclusive composite interval mapping revealed 26 stable QTL for five fatty acids, explaining 0.4–37.0% of the phenotypic variance for individual fatty acids across environments. Of these QTL, nine are novel loci (qLA1, qLNA2_1, qPA4_1, qLA4_1, qPA6_1, qSA12_1, qPA16_1, qOA18_1, and qFA19_1). These stable QTL harbor three fatty acid biosynthesis genes (GmFabG, GmACP, and GmFAD8), and 66 genes encoding lipid-related transcription factors. These stable QTL and tightly linked SNP markers can be used for marker-assisted selection in soybean breeding programs.

See: https://link.springer.com/article/10.1007/s00122-017-2902-8/fulltext.html

Figure 2: High-density genetic map for soybean and 26 stable QTL for fatty acids. The SLAF marker distributions were depicted on the 20 linkage groups (Gm01–Gm20) based on their genetic positions in centiMorgans (cM). The 26 stable QTL for fatty acids, along with their interval distances (cM) are shown between the tightly linked SLAF markers on the right side of each linkage group. The three fatty acid biosynthesis genes are indicated within the QTL intervals in red color by comparing their physical positions with that of SLAF markers in the soybean reference genome (color figure online)