Sun X, Wang Y, Xu L, Li C, Zhang W, Luo X, Jiang H, Liu L.

Front Plant Sci. 2017 Jul 14;8:1192. doi: 10.3389/fpls.2017.01192. eCollection 2017.

Abstract

To understand the molecular mechanism underlying salt stress response in radish, iTRAQ-based proteomic analysis was conducted to investigate the differences in protein species abundance under different salt treatments. In total, 851, 706, and 685 differential abundance protein species (DAPS) were identified between CK vs. Na100, CK vs. Na200, and Na100 vs. Na200, respectively. Functional annotation analysis revealed that salt stress elicited complex proteomic alterations in radish roots involved in carbohydrate and energy metabolism, protein metabolism, signal transduction, transcription regulation, stress and defense and transport. Additionally, the expression levels of nine genes encoding DAPS were further verified using RT-qPCR. The integrative analysis of transcriptomic and proteomic data in conjunction with miRNAs was further performed to strengthen the understanding of radish response to salinity. The genes responsible for signal transduction, ROS scavenging and transport activities as well as several key miRNAs including miR171, miR395, and miR398 played crucial roles in salt stress response in radish. Based on these findings, a schematic genetic regulatory network of salt stress response was proposed. This study provided valuable insights into the molecular mechanism underlying salt stress response in radish roots and would facilitate developing effective strategies toward genetically engineered salt-tolerant radish and other root vegetable crops.

See: https://www.ncbi.nlm.nih.gov/pubmed/28769938

Figure 1: Primary data analysis and protein identification. (A) Basic information statistics. (B) Number of peptides that match proteins using MASCOT. (C) Distribution of protein's sequence coverage. (D) Protein mass distribution.