ZHANG Yuanye, YIN Liying, LI Rongtian, HE Mingliang, LIU Xinxin, PAN Tingting, TIAN Xiaojie, BU Qingyun, LI Xiufeng

Chinese Journal of Rice Science ›› 2022, Vol. 36 ›› Issue (6): 572-578.

DOI: 10.16819/j.1001-7216.2022.211205

Abstract

It is of great value to restore cultivated rice varieties to red rice with good rice quality and strong stress resistance. CRISPR/Cas9 gene editing technology was used to edit the Rc gene, restoring the red seed coat, and laying a data foundation for the improvement of rice quality and resistance. Using CRISPR/Cas9 technology, the target gene Rc mutant vector pYLCRISPR/ Cas9-Rc-gRNA was constructed and transformed into transgenic plants with Kongyu 180 and Shangyu 453 as materials. The results were verified by sequencing and phenotypic observation. Two Rc mutants were obtained. For KY-1, with 4 bases deleted from 1414 bp to 1417 bp, the terminator was replaced by phenylalanine. SY-1 also lost a base at 1411 bp, which resulted in the transformation from the terminator to aspartic acid. The two kinds of editing materials restored red rice phenotype and had certain saline-alkali tolerance. Using CRISPR/Cas9 technique, we obtained homozygous lines with red seed coat, providing basic materials for the improvement of red rice.

See http://www.ricesci.cn/EN/10.16819/j.1001-7216.2022.211205

Fig. 1.   Gene structure and target site of Rc.

The black sequence is the target sequence and the gray underlined sequence is the PAM sequence.