Luo L, Wan Q, Yu Z, Zhang K, Zhang X, Zhu S, Wan Y, Ding Z, Liu F.

Int J Mol Sci. 2022 May 10;23(10):5309. doi: 10.3390/ijms23105309.

Abstract

Auxin response factors (ARFs) play important roles in plant growth and development; however, research in peanut (Arachis hypogaea L.) is still lacking. Here, 63, 30, and 30 AhARF genes were identified from an allotetraploid peanut cultivar and two diploid ancestors (A. duranensis and A. ipaensis). Phylogenetic tree and gene structure analysis showed that most AhARFs were highly similar to those in the ancestors. By scanning the whole-genome for ARF-recognized cis-elements, we obtained a potential target gene pool of AhARFs, and the further cluster analysis and comparative analysis showed that numerous members were closely related to root development. Furthermore, we comprehensively analyzed the relationship between the root morphology and the expression levels of AhARFs in 11 peanut varieties. The results showed that the expression levels of AhARF14/26/45 were positively correlated with root length, root surface area, and root tip number, suggesting an important regulatory role of these genes in root architecture and potential application values in peanut breeding.

See https://pubmed.ncbi.nlm.nih.gov/35628135/

Figure 1

Chromosome distribution of ARFs in cultivar (A) and wild (B) peanuts. Chromosomes belong to the A sub-genome of A. hypogaea (Arahy.02–10) and their homologues from A. duranensis are colored in yellow, while the chromosomes that belong to the B sub-genome of A. hypogaea (Arahy.12–20) and their homologues from A. ipaensis are colored in green. Chromosomes that do not contain ARFs and ARFs located on the scaffold are not shown.