Zhiqi Wang, Tao Liu, Wenbo Wu, Wenting Shi, Jian Shi, Fengyan Mo, Chong Du, Chaonan Wang, Zhongmin Yang. 

Plants (Basel); 2024 May 11; 13(10):1322. doi: 10.3390/plants13101322.

Abstract

Pectin is a structural polysaccharide and a major component of plant cell walls. Pectate lyases are a class of enzymes that degrade demethylated pectin by cleaving the α-1,4-glycosidic bond, and they play an important role in plant growth and development. Currently, little is known about the PL gene family members and their involvement in salt stress in potato. In this study, we utilized bioinformatics to identify members of the potato pectate lyase gene family and analyzed their gene and amino acid sequence characteristics. The results showed that a total of 27 members of the pectate lyase gene family were identified in potato. Phylogenetic tree analysis revealed that these genes were divided into eight groups. Analysis of their promoters indicated that several members' promoter regions contained a significant number of hormone and stress response elements. Further, we found that several members responded positively to salt treatment under single salt and mixed salt stress. Since StPL18 exhibited a consistent expression pattern under both single and mixed salt stress conditions, its subcellular localization was determined. The results indicated that StPL18 is localized in the endoplasmic reticulum membrane. The results will establish a foundation for analyzing the functions of potato pectate lyase family members and their expression under salt stress.

See https://pubmed.ncbi.nlm.nih.gov/38794393/

Figure 1: Genomic distribution of StPLs on potato chromosomes. Chromosome numbers are on the left and StPLs are on the right of chromosomes. Scale bar on the left indicates chromosome length.

Fig. 7: Expression patterns of the members of the potato StPL gene family under single salt and mixed salt stress. (a) Expression levels of the members of the family under single salt; (b) expression levels of the members of the StPL family under mixed salt. The color bar in the figure represents the log2 expression values, and the expression values were normalized using TPM + 1.