Genome-wide characterization of sugarcane catalase gene family identifies a ScCAT1 gene associated disease resistance |
In plants, catalase (CAT) mainly scavenges H2O2 from reactive oxygen species (ROS) and regulates the growth and development. So far, genome-wide identification of CAT gene family in Saccharum has not yet been reported. Here, 16 SsCAT genes were identified based on a Saccharum spontaneum genome. They were clustered into three subfamilies, with closer genes sharing similar structures. Most SsCAT proteins contained three conserved amino acids, one active catalytic site, one heme-ligand signature, and three peroxisomal targeting signal 1 (PTS1) sequences. The cis-regulatory element prediction revealed that SsCAT genes were involved in growth and development, and in response to various hormones and stresses. |
Qibin Wu, Yanling Chen, Wenhui Zou, Yong-Bao Pan, Peixia Lin, Liping Xu, Michael P Grisham, Qiugang Ding, Yachun Su, Youxiong Que. Int J Biol Macromol.; 2023 Mar 31; 232:123398. doi: 10.1016/j.ijbiomac.2023.123398. AbstractIn plants, catalase (CAT) mainly scavenges H2O2 from reactive oxygen species (ROS) and regulates the growth and development. So far, genome-wide identification of CAT gene family in Saccharum has not yet been reported. Here, 16 SsCAT genes were identified based on a Saccharum spontaneum genome. They were clustered into three subfamilies, with closer genes sharing similar structures. Most SsCAT proteins contained three conserved amino acids, one active catalytic site, one heme-ligand signature, and three peroxisomal targeting signal 1 (PTS1) sequences. The cis-regulatory element prediction revealed that SsCAT genes were involved in growth and development, and in response to various hormones and stresses. RNA-Seq databases showed that SsCAT genes were differentially expressed in Saccharum tissues and under cold, drought, and Sporisorium scitamineum stresses. The ScCAT1 gene transcript (GenBank accession number KF664183) and relevant CAT activity were up-regulated under S. scitamineum stress. Overexpression of ScCAT1 gene in Nicotiana benthamiana could enhance its resistance to pathogen infection through scavenging of excessive toxic ROS and up-regulated expressions of genes related to hypersensitive response (HR), ROS and salicylic acid (SA) pathways. This study provides comprehensive information for the CAT gene family and sets up a basis for its function identification in sugarcane.
See https://pubmed.ncbi.nlm.nih.gov/36702220/
Fig. 1. Chromosome location of the SsCAT gene family (A) and an unrooted phylogenetic tree of 62 CAT proteins of nine plant species (B). The nine species are depicted by different colored shapes. Gene IDs and the full sequence of 62 CAT proteins were listed in Supplemental Table S1. |
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