Feihe Chen, Haomin Zhang, Hong Li, Ling Lian, Yidong Wei, Yuelong Lin, Lanning Wang, Wei He , Qiuhua Cai, Hongguang Xie, Hua Zhang, Jianfu Zhang

BMC Plant Biol.; 2023 Jan 25;23(1):55. doi: 10.1186/s12870-023-04062-9.

Abstract

Drought is a major abiotic stress to rice (Oryza sativa) during growth. Ideal Plant Architecture (IPA1), the first cloned gene controlling the ideal plant type in rice, has been reported to function in both ideal rice plant architecture and biotic resistance. Here, we report that the IPA1/OsSPL14, encoding a transcriptional factor, positively regulates drought tolerance in rice. The IPA1 is constitutively expressed and regulated by H₂O₂, abscisic acid, NaCl and polyethylene glycol 6000 treatments in rice. Furthermore, the IPA1-knockout plants showed much greater accumulation of H₂O₂ as measured by 3,3'-diaminobenzidine staining in leaves compared with WT plants. Yeast one-hybrid, dual-luciferase and electrophoretic mobility shift assays indicated that the IPA1 directly activates the promoter of SNAC1. Expression of SNAC1 is significantly down-regulated in IPA1 knockout plants. Further investigation indicated that the IPA1 plays a positive role in drought-stress tolerance by inducing reactive oxygen species scavenging in rice. Together, these findings indicated that the IPA1 played important roles in drought tolerance by regulating SNAC1, thus activating the antioxidant system in rice.

See https://pubmed.ncbi.nlm.nih.gov/36698063/

Fig. 2: Phenotype of mature IPA1 knockout plants. A Construction of CRISPR/Cas9 vector with pHUE411. Sequence analysis of mutation sites in IPA1 knockout plants. Note:… are deleted bases; #1–#5 are knockout transgenic plants; and Target1 and Target2 are the targets for the design. Scale bar is 18 cm. B Phenotype of FH7185 and knockout transgenic plants. C Heights of FH7185 and knockout transgenic plants. D Numbers of tillers of FH7185 and knockout transgenic plants. E Flag leaf widths of FH 7185 and knockout transgenic plants. F Flag leaf lengths of FH7185 and knockout transgenic plants. G Ear lengths of FH7185 and knockout transgenic plants. Scale bar is 5 cm (H) Panicle lengths of FH7185 and knockout transgenic plants. Data are means ± se (n = 3). Statistical significance was determined by Student’s t-test. *, P < 0.05; **, P < 0.01