Rong Chen, Can Yang, Hu Gao, Chunmei Shi, Zhiying Zhang, Guangyu Lu, Xinyan Shen, Yaping Tang, Feng Li, Yongen Lu & Bo Ouyang

Theoretical and Applied Genetics October 2022; vol. 135: 3455–3468

Figure: The hypocotyl of pepper

Key message

The causal gene, CaHY5 of a chemical induced green-hypocotyl mutant was identified by molecular mapping. CaHY5 regulates anthocyanin accumulation by directly binding to the promoter of genes in anthocyanin pathway.

Abstract

Morphological markers at seedling stage are useful indicators for F₁ hybrid seeds screening. Pepper is a worldwide vegetable with diverse uses, and F₁ hybrids are popular in the pepper industry. Hypocotyl color is a useful marker to identify F₁ hybrid seeds. However, most pepper accessions have purple hypocotyl caused by anthocyanin accumulation, while green hypocotyl pepper accessions are rare. In this study, we identified a green hypocotyl mutant (e1898) from a pepper ethylmethanesulfonate (EMS) mutant library. By combining bulked segregant RNA-seq (BSR), genome resequencing and recombinant analysis, it was found that CaHY5 is the causal gene of this mutant. Virus-induced gene silencing (VIGS) of CaHY5 resulted in the decrease of anthocyanin accumulation in pepper hypocotyls. RNA-seq data showed that many genes related to anthocyanin biosynthesis and transport decreased significantly in the mutant. Yeast one-hybrid (Y1H) assays showed that CaHY5 can bind to the promoter of CaF3H, CaF3′5’H, CaDFR, CaANS and CaGST, which are important genes in anthocyanin biosynthesis or transport. Our results indicate that CaHY5 directly regulates anthocyanin biosynthesis and transport, thus governing anthocyanin accumulation in pepper hypocotyl. The mutant and gene identified in this work shall be valuable in the purity control of hybrid pepper seeds.

See https://link.springer.com/article/10.1007/s00122-022-04192-y